EMAGE mouse gene expression spatial database

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EMAGE:21335

Rcc1 regulator of chromosome condensation 1 ( MGI:1913989)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000016_01	euxassay_000016_02	euxassay_000016_03	euxassay_000016_04	euxassay_000016_05

euxassay_000016_06	euxassay_000016_07	euxassay_000016_08	euxassay_000016_09	euxassay_000016_10

euxassay_000016_11	euxassay_000016_12	euxassay_000016_13	euxassay_000016_14	euxassay_000016_15

euxassay_000016_16	euxassay_000016_17	euxassay_000016_18	euxassay_000016_19	euxassay_000016_20

euxassay_000016_21	euxassay_000016_22	euxassay_000016_23	euxassay_000016_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21335_wholemount_strong.wlz

21335_wholemount_moderate.wlz

21335_wholemount_weak.wlz

21335_wholemount_possible.wlz

21335_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21335_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 1 2

submandibular gland primordium

weak

regional

weak expression: see section 7 8 9 0

vibrissa

weak

regional

weak expression: see section 7 8 9 1

cerebral cortex mantle layer

weak

regional

weak expression: see section 2

cerebral cortex marginal layer

weak

regional

weak expression: see section 3

cerebral cortex ventricular layer

weak

regional

weak expression: see section 4 5 6 7 8 9 0 1

midbrain lateral wall

weak

regional

weak expression: see section 0 1 2

trigeminal v ganglion

weak

regional

weak expression: see section 7 8 9

mandible

weak

regional

weak expression: see section 8 0 1

palatal shelf

weak

regional

weak expression: see section 0

liver

weak

regional

weak expression: see section 3 4 5 6 7 8 9 0 1 2

metanephros

weak

regional

weak expression: see section 1

lung

weak

regional

weak expression: see section 7 8 9 0 1 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1725
Entity Detected:	Rcc1, regulator of chromosome condensation 1 ( MGI:1913989)
Sequence:	sense strand is shown >T1725 TCGAGCCAGATTCGGCACGAGGCTTCTGGNCCCAGCTCAGCTTCTCAGGACAGGAAAAGAGCCAGCTACA GATTCCAACCCTGAACAGATAGCATCTCCTGTCTTTTCCCATCACCACAGCAGAATCCTTTTTCTTCTGT TTCGTCCGTCCTCTTTCTAGAACATACTGGTACTTGTAGGATGTGTGGGGATGGGAGGAGGACCTCAGAA GGATCTTTGCTTACTTTATTAGACCTCCCCCCCTCCCCTTTGATACCCTCCTTCCTGTGGTCCTAGCTGG CACTGGCTCATTACCTACAAAACCCAAAACGGTAGCTTAAGACTACGTGCAACATCCTGCCCCGGGGTTT TGCTGCCCAGACTCTGAAAAGTTGGGACTCTGTGAAGCCCTGCTTACCACGAGCCTCTTTCTGTTCCTAA CAGTAGTTTACAAGGACACAGATGGTTTGTATTGTCATCGGATCCAGTTGTCATGGACCCATGCCTGGGG AGATCTGGACAAAGGCTTTTCAAGGCTCTGAGTGAT
Notes:	The probe template was PCR amplified from IMAGE:480014 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:480014 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000016 same experiment
	EMAGE:21332 same embryo
	EMAGE:21336 same embryo
	EMAGE:21334 same embryo
	EMAGE:21333 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source