EMAGE mouse gene expression spatial database

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EMAGE:21340

Gstm5 glutathione S-transferase, mu 5 ( MGI:1309466)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000343_01	euxassay_000343_02	euxassay_000343_03	euxassay_000343_04	euxassay_000343_05

euxassay_000343_06	euxassay_000343_07	euxassay_000343_08	euxassay_000343_09	euxassay_000343_10

euxassay_000343_11	euxassay_000343_12	euxassay_000343_13	euxassay_000343_14	euxassay_000343_15

euxassay_000343_16	euxassay_000343_17	euxassay_000343_18	euxassay_000343_19	euxassay_000343_20

euxassay_000343_21	euxassay_000343_22	euxassay_000343_23	euxassay_000343_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21340_wholemount_strong.wlz

21340_wholemount_moderate.wlz

21340_wholemount_weak.wlz

21340_wholemount_possible.wlz

21340_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21340_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 6

vibrissa

moderate

regional

moderate expression: see section 5

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 5 6 7 8 9 0 7 8 9 0 1 2 3

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 9 0 1

trigeminal v ganglion

strong

homogeneous

strong expression: see section 4 5 7 9 9 0 1 2 3 moderate expression: see section 8 4

vagus x ganglion

strong

homogeneous

strong expression: see section 9 0 9 0 moderate expression: see section 8

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 9 0 1

spinal cord

strong

homogeneous

strong expression: see section 6

labyrinth

weak

regional

weak expression: see section 6

otic capsule

moderate

regional

moderate expression: see section 5 weak expression: see section 6

nasal septum

strong

regional

strong expression: see section 5 moderate expression: see section 1

nose

strong

regional

strong expression: see section 5 6 moderate expression: see section 6 7 1

vomeronasal organ

strong

homogeneous

strong expression: see section 6

not examined

other

not examined expression: see section 5

basioccipital bone

weak

regional

weak expression: see section 4

basisphenoid bone

strong

regional

strong expression: see section 4 moderate expression: see section 7 2 3

sphenoid bone

moderate

regional

moderate expression: see section 3 4

orbito-sphenoid

moderate

regional

moderate expression: see section 5

tail central nervous system

strong

homogeneous

strong expression: see section 3 4

tail dorsal root ganglion

strong

homogeneous

strong expression: see section 2 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1684
Entity Detected:	Gstm5, glutathione S-transferase, mu 5 ( MGI:1309466)
Sequence:	sense strand is shown >T1684 TGGCCTCGAGCCAGATTCGGCACGAGGGATCGCCCAAAATGTCATCCAAGTCTATGGTTCTGGGTTACTG GGATATCCGCGGGCTGGCTCATGCTATCCGCATGCTTCTGGAGTTTACTGATACCAGCTATGAGGAGAAA CGGTACATCTGTGGGGAAGCTCCTGACTATGATAGAAGCCAATGGCTGGACGTGAAATTCAAGCTAGATC TGGACTTTCCTAACCTGCCCTACCTCATGGACGGGAAGAACAAGATCACCCAGAGTAACGCCATCCTGAG ATACATCGCACGCAAGCACAACATGTGTGGTGACACTGAAGAAGAAAAGATACGAGTAGACATCATGGAG AACCAGATCATGGACTTCCGCATGCAGCTGGTTCGCCTCTGCTACAATTCTAACCACGAAAACCTGAAGC CTCAGTACTTGGAACAGCTACCTGCACAGCTGAAACAATTCTCATTGTTCCTGGGGAAATTCACATGGTT TGCAGGAGAAAAGCTGACCTTTGTGGATTTTCTCACCTATGATGTCTTGGATCAGAACCGTATATTTGAG CCCAAG
Notes:	The probe template was PCR amplified from IMAGE:475690 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:475690 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000343 same experiment
	EMAGE:21338 same embryo
	EMAGE:21341 same embryo
	EMAGE:21337 same embryo
	EMAGE:21339 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source