EMAGE mouse gene expression spatial database

EMAGE:21341

Hrc histidine rich calcium binding protein ( MGI:96226)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000168. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000168_01	euxassay_000168_02	euxassay_000168_03	euxassay_000168_04

euxassay_000168_05	euxassay_000168_06	euxassay_000168_07	euxassay_000168_08	euxassay_000168_09

euxassay_000168_10	euxassay_000168_11	euxassay_000168_12	euxassay_000168_13	euxassay_000168_14

euxassay_000168_15	euxassay_000168_16	euxassay_000168_17	euxassay_000168_18	euxassay_000168_19

euxassay_000168_20	euxassay_000168_21	euxassay_000168_22	euxassay_000168_23	euxassay_000168_24

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1678
Entity Detected:	Hrc, histidine rich calcium binding protein ( MGI:96226)
Sequence:	sense strand is shown >T1678 TGGCCTCGAGCCAGATTCGGACGAGGCTGCGAGAGAGGTGGCCAGAGAAGGTTCCAGTGAAGAGGAGAGC CGTGAGGTCACAGGTCAGCAGGATGCCCAGGAGTACGAAAATTACCAGCCAGGGTCTTTGTGTGGCTACT GTTCTTTCTGCAACCGATGTACCGAATGTGAAAGCTGTCACTGTGATGAGGAGAACATGGGGGAACACTG TGACCAGTGTCAGCACTGTCAATTCTGCTACCTCTGTCCGCTGGTGTGTGACACGCTCTGCACTCCAGGA AGCTACGTTGACTATTTCTCCTCCTCTCTGTATCAAGCCCTGGCTGACATGTTGGAGACTCCAGAGCCCT GACCTGGCCGCCTGGCAAGAGCTGCATCTATTTCTTTGAATAAATGTGCTCCTAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:475063 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:475063 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000168 same experiment
	EMAGE:21338 same embryo
	EMAGE:21337 same embryo
	EMAGE:21340 same embryo
	EMAGE:21339 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source