EMAGE mouse gene expression spatial database

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EMAGE:21389

Pdia4 protein disulfide isomerase associated 4 ( MGI:104864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000803_01	euxassay_000803_02	euxassay_000803_03	euxassay_000803_04	euxassay_000803_05

euxassay_000803_06	euxassay_000803_07	euxassay_000803_08	euxassay_000803_09	euxassay_000803_10

euxassay_000803_11	euxassay_000803_12	euxassay_000803_13	euxassay_000803_14	euxassay_000803_15

euxassay_000803_16	euxassay_000803_17	euxassay_000803_18	euxassay_000803_19	euxassay_000803_20

euxassay_000803_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21389_wholemount_strong.wlz

21389_wholemount_moderate.wlz

21389_wholemount_weak.wlz

21389_wholemount_possible.wlz

21389_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21389_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

not examined

homogeneous

not examined expression: see section 1 2 3 4 5 6 7 8 0 1 2 4 5 6 7 8 9 0

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 0 1 5 6

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

medulla oblongata basal plate

moderate

regional

moderate expression: see section 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1045
Entity Detected:	Pdia4, protein disulfide isomerase associated 4 ( MGI:104864)
Sequence:	sense strand is shown >T1045 CTCGAGNCTGTTGGCCTACTGGAGTGCTCTGCGGTGGCCGGTGTCTGCAGGCCCAGCTCTGCACCCAATC ATGAAGCTCCGGAAAGCTTGGCTGTTGGTCCTGCTCTTGGCGCTGACACAGCTGCTGGCTGCTGCGAGCG CCGGAGATGCACACGAAGATACTTCAGATACAGAAAATGCCACTGAGGAGGAAGAGGAGGAAGAGGAAGA GGATGACGATGACTTGGAAGTTAAGGAAGAAAATGGTGTTTGGGTCTTAAATGATGGGAACTTTGATAAC TTTGTGGCTGACAAAGATACAGTGCTACTGGAGTTCTATGCACCATGGTGTGGACACTGCAAGCAGTTTG CTCCAGAATATGAGAAAATTGCCAGTACTTTGAAGGATAATGATCCTCCCATTGCTGTAGCGAAGATCGA TGCAACCTCAGCATCCATGCTAGCCAGCAAATTTGATGTGAGTGGCTACCCCACCATCAAGATCCTGAAG AAGGGACAGGCCGTTGACTATGATGGCTCCAGGACCCAGGAAGAAATTGTTGCCAAAGTCAGAGAAGTTT CCCAGCC
Notes:	The probe template was PCR amplified from IMAGE:2088217 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088217 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000803 same experiment
	EMAGE:21390 same embryo
	EMAGE:21391 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source