EMAGE mouse gene expression spatial database

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EMAGE:21402

Aqp1 aquaporin 1 ( MGI:103201)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_018969_01	euxassay_018969_02	euxassay_018969_03	euxassay_018969_04	euxassay_018969_05

euxassay_018969_06	euxassay_018969_07	euxassay_018969_08	euxassay_018969_09	euxassay_018969_10

euxassay_018969_11	euxassay_018969_12	euxassay_018969_13	euxassay_018969_14	euxassay_018969_15

euxassay_018969_16	euxassay_018969_17	euxassay_018969_18	euxassay_018969_19	euxassay_018969_20

euxassay_018969_21	euxassay_018969_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21402_wholemount_strong.wlz

21402_wholemount_moderate.wlz

21402_wholemount_weak.wlz

21402_wholemount_possible.wlz

21402_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21402_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 6 7 8 0 1 2

humerus

moderate

regional

moderate expression: see section 3 4 5 1 2 weak expression: see section 2 6

hand mesenchyme

moderate

regional

moderate expression: see section 1 2 3 4 5 6 2

foot mesenchyme

moderate

regional

moderate expression: see section 3 5 6 7 9 1 2

femur

weak

regional

weak expression: see section 7 8 9 9 0 1 2

vibrissa

strong

regional

strong expression: see section 4 5 6 7 1 2 moderate expression: see section 0

lateral ventricle choroid plexus

strong

regional

strong expression: see section 5 6 7 8 9 0 3 4 5 6 7 8 9

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1

otic capsule

weak

regional

weak expression: see section 8 9 5 6 7

naris

moderate

regional

moderate expression: see section 1 2 6 7

nasal septum

moderate

regional

moderate expression: see section 2 3 4 5 6 7

nose

moderate

regional

moderate expression: see section 9 0 1 5 6 7 8 9 0 weak expression: see section 7 8

heart valve

moderate

regional

moderate expression: see section 1 2 3

mandible

weak

regional

weak expression: see section 4 5 6 7 9 0 7 8 9 0 1 2

meckel's cartilage

moderate

regional

moderate expression: see section 0 1 2 3 4 5 6 7 weak expression: see section 7 8 9 8 9

maxilla

weak

regional

weak expression: see section 7

liver

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2

male reproductive system

moderate

regional

moderate expression: see section 2 5

thyroid cartilage

weak

regional

weak expression: see section 1 2 3 4

axial skeleton

moderate

regional

moderate expression: see section 8 9 0 1 2 3 4 5 6 7

orbito-sphenoid

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 8 9 0 1 2

clavicle

weak

regional

weak expression: see section 9 0 2 3 4 6 7

pelvic girdle skeleton

moderate

regional

moderate expression: see section 9 0 8 weak expression: see section 7 9

tail mesenchyme

moderate

regional

moderate expression: see section 7

axial skeleton tail region

weak

regional

weak expression: see section 4 5 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50113
Entity Detected:	Aqp1, aquaporin 1 ( MGI:103201)
Sequence:	sense strand is shown >T50113 GTTGAGCACCAGGCATCCAGCGGTCAAGTCGAGGCCCCTGCCAGCATGGCCAGTGAAATCAAGAAGAAGC TCTTCTGGAGGGCTGTGGTGGCTGAGTTCCTGGCCATGACCCTCTTCGTCTTCATCAGCATCGGTTCTGC CCTAGGCTTCAATTACCCACTGGAGAGAAACCAGACGCTGGTCCAGGACAACGTGAAGGTGTCGCTGGCC TTTGGTTTGAGCATCGCTACTCTGGCCCAAAGTGTGGGTCACATCAGCGGTGCTCACCTCAACCCTGCGG TCACACTGGGGCTCCTGCTCAGCTGTCAGATCAGCATCCTCCGGGCTGTCATGTACATCATCGCCCAGTG TGTGGGAGCCATCGTCGCCACGGCCATTCTCTCGGGCATCACCTCCTCCCTAGTCGACAATTCACTTGGC CGCAATGACCTGGCTCACGGTGTGAACTCCGGCCAAGGCCTGGGCATTGAGATCATTGGCACTCTGCAGC TGGTACTGTGCGTTCTGGCCACCACTGACCGGAGGCGCCGAGACTTAGGTGGCTCAGCCCCGCTTGCCAT TGGCTTGTCTGTGGCCCTTGGACACCTGCTGGCGATTGACTACACTGGCTGCAGTATCAACCCTGCCCGG TCATTTGGCTCTGCTGTGCTCACCCGCAACTTCTCAAACCACTGGATTTTCTGGGTGGGGCCGTTCATTG GGGGTGCCCTGGCAGTGCTCATCTATGACTTCATCCTGGCCCCACGCAGCAGCGACTTCACAGACCGCAT GAAGGTGTGGACCAGTGGCCAGGTGGAGGAGTATGACCTGGATGCTGACGACATCAACTCCAGGGTGGAG ATGAAGCCCAAATAGAGGAGGCTTGGCCTGGGCATCTATATGGGGGTGGGGCAGGGATGGGCGGAGGGAG GGGAGGGCCGAAATCATATTGTAGACACTCTGACAAGCTGACCAAAGTCACTCCCCAAGACCTGCCTGAC CCACATGGTCTAGCCTTGTCTGGGGCAGTAATATCACTGTCTTTCTTTCTGTTTCCTGGTCTCAGAGCTT CCTGGGGACCAAGATTTATCAACTCAGCACCCCACTCCTTTGACATCATGGAGGAGTGAAAGCGAGGGAC CCACTTGCTAGTTGTCCCCTCAGAGTGTGCTAGGAAGTCCCCCTCATTCCAAAGCTGCCCAGCAAGTCCC CCACCTTAGGTGCCTGGGATTCTGCCAATGTCGCTTTGTGCCTTTGACCGCAGCCTTCCTTGGGGTAGGC ACCTTATCTCCAAAGGCACTTTGAGGGAGAGGAGGTCCCCTAACTTTCCCCTTTGGTCTGACTTACCTCC AGGACCCTTCCCCTTGAACTCACTCTAAGACCTTGGAATTGTCCCTGTGCTGAGGCCTCAGTGATCACAT CTGTAAAGTGGCAAGGAAGGGACAGCTTTGGGGATTCTGTAAGTGTGGACATGCCACAGGTTCTAGAAGG GACAGTCTTGACAGCACTGCTTTGTGTACCCGGCCTATACTCTTTCCCAGCGTCCACAGCCCACGAGCAC ACGTGGATGGGCCACGGATCAGAGAATCAGTGTGGGATGCAAGACGTGCCTGTTCACACACACAGCCTTC CTAGAGGCGGGTTAGCTGTAGCAGAACGAAGGTCATTTTGTGGTAGAAGAAGAGCTCACACTATCTTACC TAGCTCTAGTGGGTCTGCCCGTGTAACATGGCACCGACTGGATGTTTATTTCGGTCCTCCAGTCTCCCTT GCAGTATAGATCTGGTGTGTCTGTGTTTGTTAAAGAGCATTGGGCTATGTGCAGTGTCATGTCTGAGGAC ATGCTGGGCACACAAGGGTTTGGATGCCCTTAGTTCATCATGAGCAAGGAGGTCACTCTGGCCCTGGCTT GTGAGCTCCTGGCTGGATGAGTGTGACTGGAGCCTGGACAATCTGCAGAGGCTTACTCTGTGCCTCCAGG CACAGTCTTCTCTCTGCATTGGCCCAATGCTACAGCTTAT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_018969 same experiment
	EMAGE:21400 same embryo
	EMAGE:21399 same embryo
	EMAGE:21401 same embryo
	EMAGE:21398 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source