EMAGE mouse gene expression spatial database

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EMAGE:21403

D10Wsu52e DNA segment, Chr 10, Wayne State University 52, expressed ( MGI:106379)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000797_01	euxassay_000797_02	euxassay_000797_03	euxassay_000797_04	euxassay_000797_05

euxassay_000797_06	euxassay_000797_07	euxassay_000797_08	euxassay_000797_09	euxassay_000797_10

euxassay_000797_11	euxassay_000797_12	euxassay_000797_13	euxassay_000797_14	euxassay_000797_15

euxassay_000797_16	euxassay_000797_17	euxassay_000797_18	euxassay_000797_19	euxassay_000797_20

euxassay_000797_21	euxassay_000797_22	euxassay_000797_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21403_wholemount_strong.wlz

21403_wholemount_moderate.wlz

21403_wholemount_weak.wlz

21403_wholemount_possible.wlz

21403_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21403_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3932
Entity Detected:	D10Wsu52e, DNA segment, Chr 10, Wayne State University 52, expressed ( MGI:106379)
Sequence:	sense strand is shown >T3932 CCCACGCGTCCGGCGTGGAGCGGGTGGACTGCGGGGTGGTTTCTCTTTCTAAGCGTAGCCAGGAGCTATC GCTGGCCTCGCCGCCACCATGAGTCGTAACTACAACGATGAGCTACAGTTCTTGGACAAGATCAATAAAA ACTGCTGGAGGATCAAGAAGGGCTTTGTGCCCAACATGCAGGTTGAAGGAGTGTTTTATGTGAATGATGC TCTGGAAAAACTAATGTTTGAGGAATTAAGGAACGCCTGTCGAGGTGGTGGTGTTGGTGGCTTTCTGCCA GCCATGAAGCAGATTGGCAATGTGGCAGCCCTGCCTGGAATAGTTCATCGGTCTATCGGGCTTCCTGATG TCCATTCAGGCTATGGGTTTGCCATAGGGAACATGGCTGCCTTTGATATGAATGACCCTGAGGCCGTTGT ATCCCCAGGTGGTGTCGGATTTGATATTAACTGTGGTGTCCGCTTGCTAAGAACCAATTTAGATGAGAGC GATGTACAGCCTGTGAAGGAACAACTTGCCCAAGCTATGTTTGACCACATCCCTGTTGGGGTGGGATCAA AAGGTGTCATTCCAATGAATGCCAAAGACTTGGAGGAGGCATTGGAGATGGGGGTGGACTGGTCCCTGAG GGAAGGCTATGCCTGGGCTGAAGACAAGGAGCACTGTGAGGAGTATGGAAGGATGCTGCAAGCCGACCCC AATAAGGTCTCACCCAGGGCAAAGAAAAGGGGCCTTCCTCAGTTGGGGACCCTGGGAGCAGGCAACCATT ATGCAGAAATCCAGGTTGTAGATGAGATTTTCAATGAGTATGCCGCCAAGAAGATGGGCATCGACCATAA GGGACAGGTGTGTGTGATGATCCACAGTGGAAGCAGAGGCTTGGGCCACCAAGTAGCTACAGATGCACTG GTAGCTATGGAAAAGGCCATGAAGAGAGACAAGATTATAGTCAATGACCGGCAGTTGGCTTGTGCTCGGA TTGCATCCCCAGAGGGACAAGACTATCTAAAGGGAATGGCTGCAGCTGGAAACTACGCCTGGGTTAACCG CTCCTCTATGACCTTCTTAACCCGTCAGGCTTTTGCCAAAGTCTTCAACACAACCCCTGATGACCTGGAC CTGCATGTGATCTATGATGTGTCGCACAATATCGCCAAAGTGGAGCAGCACGTGGTGGATGGGAAGGAAC GGACGCTGCTGGTGCACAGGAAGGGATCCACCCGTGCTTTCCCGCCTCACCACCCCCTCATTGCTGTGGA TTATCAACTCACAGGACAACCAGTGCTTATTGGTGGCACCATGGGGACCTGTAGTTACGTTCTGACTGGC ACTGAACAAGGCATGACTGAGACCTTTGGAACAACCTGTCATGGAGCGGGCCGTGCTTTGTCCAGAGCAA AATCACGTCGTAACTTAGATTTCCAAGATGTCTTAGACAAACTGGCAGACATGGGAATTGCAATCCGGGT TGCTTCCCCCAAGCTGGTTATGGAAGAGGCACCAGAGTCCTATAAGAATGTGACAGACGTCGTGAACACC TGCCATGATGCTGGGATCAGCAAGAAGGCCATTAAACTGAGACCAATTGCTGTTATTAAAGGGTAGATCC AAGACTATCAGAGCGTCTGAGGTGCCCATGGACTGAAATTCCTAAGACATGAGACTCAAGGCCTGACAAG ATCCGAATTGCAGAGCTGTTGCTGTGCGTGCCACCATGGCTGGTGGAGAGGCTGCTGTCACAGGGTTGGA AAATAGCCTTCCAGGAGATGTGTCTCTGATGGGAGCAGGGCAAATGAGCCTCTTCTTCCTTTGTCCCACA GGGGTTTGTGACTGTCAATTGGGGTGTGGGAAGGGGCCCAGAAATGCCCTGTTTGGTCATACAAGTCTGT AATCACCAGAGTGGGCTTCTTTATCCCAGGCCCTGTTTGTAGCCCAGAAGGTATCATGGAGAACATGCTA TTAAATAGGTTTTTGAATGTTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3583081 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3583081 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000797 same experiment
	EMAGE:21404 same embryo
	EMAGE:21405 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source