EMAGE mouse gene expression spatial database

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EMAGE:21405

Ctgf connective tissue growth factor ( MGI:95537)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000815_01	euxassay_000815_02	euxassay_000815_03	euxassay_000815_04	euxassay_000815_05

euxassay_000815_06	euxassay_000815_07	euxassay_000815_08	euxassay_000815_09	euxassay_000815_10

euxassay_000815_11	euxassay_000815_12	euxassay_000815_13	euxassay_000815_14	euxassay_000815_15

euxassay_000815_16	euxassay_000815_17	euxassay_000815_18	euxassay_000815_19	euxassay_000815_20

euxassay_000815_21	euxassay_000815_22	euxassay_000815_23	euxassay_000815_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21405_wholemount_strong.wlz

21405_wholemount_moderate.wlz

21405_wholemount_weak.wlz

21405_wholemount_possible.wlz

21405_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21405_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

alimentary system

moderate

regional

moderate expression: see section 6 8 9 0 weak expression: see section 4 5

arch of aorta

strong

regional

strong expression: see section 4 5

thoracic aorta

strong

regional

strong expression: see section 4 5

descending thoracic aorta

strong

regional

strong expression: see section 1 2 3

descending aorta

strong

regional

strong expression: see section 4 5

carotid artery

strong

regional

strong expression: see section 7 8

pulmonary artery

strong

regional

strong expression: see section 3 4

pulmonary trunk

strong

regional

strong expression: see section 3 4

umbilical artery embryonic part

strong

regional

strong expression: see section 7 8 9

left ventricle cardiac muscle

strong

single cell

strong expression: see section 6 7

right ventricle cardiac muscle

strong

single cell

strong expression: see section 9 0 1

stomach

moderate

regional

moderate expression: see section 4 5 6 7 8 9 0

respiratory system cartilage

strong

regional

strong expression: see section 4 5

larynx

strong

regional

strong expression: see section 4 5

respiratory tract

strong

regional

strong expression: see section 4 5

skeleton

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 2 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3933
Entity Detected:	Ctgf, connective tissue growth factor ( MGI:95537)
Sequence:	sense strand is shown >T3933 GAAGACTCAGCCAGATCCACTCCAGCTCCGACCCCAGGAGACCGACCTCCTCCAGACGGCAGCAGCCCCA GCCCAGCCGACAACCCCAGACGCCACCGCCTGGAGCGTCCAGACACCAACCTCCGCCCCTGTCCGAATCC AGGCTCCGGCCGCGCCTCTCGTCGCCTCTGCACCCTGCTGTGCATCCTCCTACCGCGTCCCGATCATGCT CGCCTCCGTCGCAGGTCCCATCAGCCTCGCCTTGGTGCTCCTCGCCCTCTGCACCCGGCCTGCTACGGGC CAGGACTGCAGCGCGCAATGTCAGTGCGCAGCCGAAGCAGCGCCGCACTGCCCCGCCGGCGTGAGCCTGG TGCTGGACGGCTGCGGCTGCTGCCGCGTCTGCGCCAAGCAGCTGGGAGAACTGTGTACGGAGCGTGACCC CTGCGACCCACACAAGGGCCTCTTCTGCGATTTCGGCTCCCCCGCCAACCGCAAGATCGGAGTGTGCACT GCCAAAGATGGTGCACCCTGTGTCTTCGGTGGGTCGGTGTACCGCAGCGGTGAGTCCTTCCAAAGCAGCT GCAAATACCAATGCACTTGCCTGGATGGGGCCGTGGGCTGCGTGCCCCTGTGCAGCATGGACGTGCGCCT GCCCAGCCCTGACTGCCCCTTCCCGAGAAGGGTCAAGCTGCCTGGGAAATGCTGCGAGGAGTGGGTGTGT GACGAGCCCAAGGACCGCACAGCAGTTGGCCCTGCCCTAGCTGCCTACCGACTGGAAGACACATTTGGCC CAGACCCAACTATGATGCGAGCCAACTGCCTGGTCCAGACCACAGAGTGGAGCGCCTGTTCTAAGACCTG TGGGATGGGCATCTCCACCCGAGTTACCAATGACAATACCTTCTGCAGACTTGAGAAGCAGAGTCGCCTC TGCATGGTCAGGCCCTGCGAAGCTGACCTGGAGGAAAACATTAAGAAGGGCAAAAAGTGCATCCGGACAC CTAAAATCGCCAAGCCTGTCAAGTTTGAGCTTTCTGGCTGCACCAGTGTGAAGACATACAGGGCTAAGTT CTGCGGGGTGTGCACAGACGGCCGCTGCTGCACACCGCACAGAACCACCACTCTGCCAGTGGAGTTCAAA TGCCCCGATGGCGAGATCATGAAAAAGAATATGATGTTCATCAAGACCTGTGCCTGCCATTACAACTGTC CTGGGGACAATGACATCTTTGAGTCCCTGTACTACAGGAAGATGTACGGAGACATGGCGTAAAGCCAGGA AGTAAGGGACACGAACTCATTAGACTATAACTTGAACTGAGTTGCATCTCATTTTCTTCTGTAAAAACAA TTACAGTAGCACATTAATTTAAATCTGTGTTTTTAACTACCGTGGGAGGAACTATCCCACCAAAGTGAGA ACGTTATGTCATGGCCATACAAGTAGTCTGTCAACCTCAGACACTGGTTTCGAGACAGTTTACACTTGAC AGTTGTTCATTAGCGCACAGTGCCAGAACGCACACTGAGGTGAGTCTCCTGGAACAGTGGAGATGCCAGG AGAAAGAAAGACAGGTACTAGCTGAGGTTATTTTAAAAGCAGCAGTGTGCCTACTTTTTGGAGTGTAACC GGGGAGGGCAATTATAGCATGCTTGCAGACAGACCTGCTCTAGCGAGAGCTGAGCATGTGTCCTCCACTA GATGAGGCTGAGTCCAGCTGTTCTTTAAGAACAGCAGTTTCAGCTCTGACCATTCTGATTCCAGTGACAC TTGTCAGGAGTCAGAGCCTTGTCTGTTAGACTGGACAGCTTGTGGCAAGTAAGTTTGCCTGTAACAAGCC AGATTTTTATTGATATTGTAAATATTGTGGATATATATATATATATATTTGTACAGTTATCTAAGTTAAT TTAAAGTCATTTGTTTTTGTTTTAAGTGCTTTTGGGATTTTAAACTGATAGCCTCAAACTCCAAACACCA TAGGTAGGACACGAAGCTTATCTGTGATTCAAAACAAAGGAGATACTGCAGTGGGAATTGTGACCTGAGT GACTCTCTGTCAGAACAAATGCTGTGCAGGTGATAAAGCTATGTATTGGAAGTCAGATTTCTAGTAGGAA ATGTGGTCAAATCCCTGTTGGTGAACAAATGGCCTTTATTAAGAAATGGCTGGCTCAGGGTAAGGTCCGA TTCCTACCAGGAAGTGCTTGCTGCTTCTTTGATTATGACTGGTTTGGGGTGGGGGGCAGTTTATTTGTTG AGAGTGTGACCAAAAGTTACATGTTTGCACCTTTCTAGTTGAAAATAAAGTATATATATATTTTTTATAT GAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589136 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589136 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000815 same experiment
	EMAGE:21403 same embryo
	EMAGE:21404 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source