EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21415

Cdc20 cell division cycle 20 homolog (S. cerevisiae) ( MGI:1859866)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000814_01	euxassay_000814_02	euxassay_000814_03	euxassay_000814_04	euxassay_000814_05

euxassay_000814_06	euxassay_000814_07	euxassay_000814_08	euxassay_000814_09	euxassay_000814_10

euxassay_000814_11	euxassay_000814_12	euxassay_000814_13	euxassay_000814_14	euxassay_000814_15

euxassay_000814_16	euxassay_000814_17	euxassay_000814_18	euxassay_000814_19	euxassay_000814_20

euxassay_000814_21	euxassay_000814_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21415_wholemount_strong.wlz

21415_wholemount_moderate.wlz

21415_wholemount_weak.wlz

21415_wholemount_possible.wlz

21415_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21415_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 8 2 moderate expression: see section 9 0 1

submandibular gland primordium

strong

regional

strong expression: see section 5 6 7 3 5 6 7 moderate expression: see section 4

hypothalamus

strong

regional

strong expression: see section 8 9 0 2

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 9 0

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 3 4 5

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

rest of cerebellum ventricular layer

strong

homogeneous

strong expression: see section 5 6 7 8

metencephalon rest of alar plate ventricular layer

strong

homogeneous

strong expression: see section 2 4 5 4 5

midbrain ventricular layer

strong

homogeneous

strong expression: see section 5 6 7 8 9 0 1 2 not examined expression: see section 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3864
Entity Detected:	Cdc20, cell division cycle 20 homolog (S. cerevisiae) ( MGI:1859866)
Sequence:	sense strand is shown >T3864 TGGCCTCGAGCCAGATTCGGACGAGGCATCANAAAGCCTGGTCTCTCAACCTGAACGGTTTTGATGTGGA GGAAGCCAAGATCCTCAGGCTCAGCGGCAAACCTCAGAATGCCCCAGAAGGCTACCAGAACAGACTGAAA GTACTGTACAGTCAGAAAGCCACGCCTGGCTCCAGCAGGAAGACTTGCAGATACATCCCTTCTCTGCCAG ACAGGATCCTTGATGCCCCCGAAATCCGGAATGACTACTACCTGAATCTTGTAGATTGGAGCTCTGGAAA TGTATTGGCTGTGGCACTGGACAACAGTGTGTACTTATGGAACGCTGGTTCTGGTGACATCCTGCAGTTG TTGCAAATGGAGCAGCCTGGAGACTACATATCCTCGGTGGCCTGGATCAAGGAGGGCAACTACCTAGCTG TAGGCACCAGCAATGCTGAGGTCCAGCTCTGGGATGTGCAGCAGCAGAAACGACTTCGAAACATGACCAG TCACTCCGCTCGAGTAAGCTCCCTCAGTTGGAACAGCTATATCCTATCCAGTGGTTCACGGTCTGGCCAC
Notes:	The probe template was PCR amplified from IMAGE:425838 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:425838 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000814 same experiment
	EMAGE:21410 same embryo
	EMAGE:21413 same embryo
	EMAGE:21414 same embryo
	EMAGE:21411 same embryo
	EMAGE:21412 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source