EMAGE mouse gene expression spatial database

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EMAGE:21416

Ppp2r1a protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform ( MGI:1926334)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000885_01	euxassay_000885_02	euxassay_000885_03	euxassay_000885_04	euxassay_000885_05

euxassay_000885_06	euxassay_000885_07	euxassay_000885_08	euxassay_000885_09	euxassay_000885_10

euxassay_000885_11	euxassay_000885_12	euxassay_000885_13	euxassay_000885_14	euxassay_000885_15

euxassay_000885_16	euxassay_000885_17	euxassay_000885_18	euxassay_000885_19	euxassay_000885_20

euxassay_000885_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21416_wholemount_strong.wlz

21416_wholemount_moderate.wlz

21416_wholemount_weak.wlz

21416_wholemount_possible.wlz

21416_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21416_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

homogeneous

moderate expression: see section 3 4 5 8

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 5 6 6

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 5 6 6

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 3 4 5 6 7 8 9 5 6 7 8 9 0

vagus x ganglion

moderate

homogeneous

moderate expression: see section 7 8 5

vestibulocochlear viii ganglion vestibular component

moderate

homogeneous

moderate expression: see section 5 7

dorsal root ganglion

moderate

regional

moderate expression: see section 7 8 9 0 1 4 5 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1042
Entity Detected:	Ppp2r1a, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform ( MGI:1926334)
Sequence:	sense strand is shown >T1042 GTTGGCCTACTGGACCGCCCCGCCCGATCCCGGATTCCGCCTGGAGCTCGCTGCTGCCTGATAGAAACGC CAGGGAGCCAAGATGGCAGCTGCCGACGGTGACGATTCGCTCTATCCTATTGCGGTGCTCATAGATGAAC TCCGCAATGAAGACGTTCAGCTTCGTCTCAATAGTATCAAGAAGCTCTCCACAATTGCCTTGGCCCTTGG GGTTGAACGGACCAGAAGTGAGCTCCTGCCCTTCCTTACAGATACCATTTATGATGAAGATGAGGTCCTC TTGGCCTTGGCTGAACAGCTGGGAACCTTCACAACTTTGGTGGGAGGGCCTGAGTATGTGCACTGTCTGC TGCCACCCCTTGAGTCACTGGCCACAGTGGAAGAGACAGTAGTGCGAGACAAGGCGGTAGAATCCTTGCG GGCCATCTCTCATGAGCACTCACCTTCCGATCTAGAGGCTCACTTTGTGCCTCTGGTAAAGCGGCTGGCG GGTGGAGACTGGTTCACCTCCCGCACCTCGGCCTGTGGTCTCTTCTCAGTTTGCTACCCCCGAGTATCCA GTG
Notes:	The probe template was PCR amplified from IMAGE:2088179 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088179 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000885 same experiment
	EMAGE:21420 same embryo
	EMAGE:21419 same embryo
	EMAGE:21417 same embryo
	EMAGE:21418 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source