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EMAGE:21417

Casp14 caspase 14 ( MGI:1335092)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21417 EMAGE:21417 EMAGE:21417 EMAGE:21417 EMAGE:21417
euxassay_000813_01 euxassay_000813_02 euxassay_000813_03 euxassay_000813_04 euxassay_000813_05
EMAGE:21417 EMAGE:21417 EMAGE:21417 EMAGE:21417 EMAGE:21417
euxassay_000813_06 euxassay_000813_07 euxassay_000813_08 euxassay_000813_09 euxassay_000813_10
EMAGE:21417 EMAGE:21417 EMAGE:21417 EMAGE:21417 EMAGE:21417
euxassay_000813_11 euxassay_000813_12 euxassay_000813_13 euxassay_000813_14 euxassay_000813_15
EMAGE:21417 EMAGE:21417 EMAGE:21417 EMAGE:21417 EMAGE:21417
euxassay_000813_16 euxassay_000813_17 euxassay_000813_18 euxassay_000813_19 euxassay_000813_20
EMAGE:21417
euxassay_000813_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21417Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21417_wholemount_strong.wlz
21417_wholemount_moderate.wlz
21417_wholemount_weak.wlz
21417_wholemount_possible.wlz
21417_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21417_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1040
Entity Detected:Casp14, caspase 14 ( MGI:1335092)
Sequence:sense strand is shown

>T1040
GCTCTAACTCCTCTTGAATGACAGCGCTGTTATCAGCAGCTTGGAGACAGACAAGCCAGGGCCTGATCCC
AAGGAGAAGGAGCACCTGCTTCTACAGCCGGGCAAAACAAAGGTGCTGAAAGCCAGACATGGAGTCAGAG
ATGAGAGATCCTCAGCCATTGCAGGAGGAAAGATATGATATGTCAGGTGCCCGCCTGGCCCTGACGCTGT
GTGTCACCAAAGCCCGGGAGGGTTCCGAGGTAGACATGGAGGCCCTGGAACGCATGTTCCGTTACCTGAA
ATTTGAAAGCACCATGAAGAGGGATCCCACCGCCCAGCAATTTCTGGAAGAGTTGGATGAATTTCAGCAG
ACCATAGATAATTGGGAAGAGCCTGTCAGCTGTGCCTTTGTGGTACTCATGGCGCATGGTGAGGAAGGCC
TCCTCAAGGGAGAAGATGAGAAGATGGTCAGACTAGAAG
Notes:The probe template was PCR amplified from IMAGE:2088160 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088160 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000813 same experiment
 EMAGE:21420 same embryo
 EMAGE:21419 same embryo
 EMAGE:21416 same embryo
 EMAGE:21418 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS