EMAGE mouse gene expression spatial database

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EMAGE:21430

Prkcd protein kinase C, delta ( MGI:97598)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000773_01	euxassay_000773_02	euxassay_000773_03	euxassay_000773_04	euxassay_000773_05

euxassay_000773_06	euxassay_000773_07	euxassay_000773_08	euxassay_000773_09	euxassay_000773_10

euxassay_000773_11	euxassay_000773_12	euxassay_000773_13	euxassay_000773_14	euxassay_000773_15

euxassay_000773_16	euxassay_000773_17	euxassay_000773_18	euxassay_000773_19	euxassay_000773_20

euxassay_000773_21	euxassay_000773_22	euxassay_000773_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21430_wholemount_strong.wlz

21430_wholemount_moderate.wlz

21430_wholemount_weak.wlz

21430_wholemount_possible.wlz

21430_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21430_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate

weak

homogeneous

weak expression: see section 4 5 6 2 3 4

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 4 weak expression: see section 5

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 4 weak expression: see section 5

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 1 2 3 4 4 6 7 8 9 weak expression: see section 5 6 5

superior vagus x ganglion

moderate

homogeneous

moderate expression: see section 4 weak expression: see section 5

vestibulocochlear viii ganglion cochlear component

weak

homogeneous

weak expression: see section 4 5 6

vestibulocochlear viii ganglion vestibular component

moderate

homogeneous

moderate expression: see section 2 3 7 weak expression: see section 4 5 6

dorsal root ganglion

moderate

regional

moderate expression: see section 4 5 6 7 8 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3837
Entity Detected:	Prkcd, protein kinase C, delta ( MGI:97598)
Sequence:	sense strand is shown >T3837 TGGCCTCGAGGCCAGATTCGGCACGAGGCGACTCTATGGACCAGGAAGCCCTCCATGGCTTCTCCTTTGT GAATCCCAAGTTTGAGCAATTCCTGGACATTTAATTAAGCTCCCAGACCTGCTTTAAACGCCCTGGCAGA GTAGGCCCGTCTGCCCTGGTTTGCACCGCATTGCCCGTGGAGAACAGTGGGTGACTGGCTGATTTCTGCT GCCACCCCCTCCTCCTTGGAGAGTCCGGCTCCTGCTGGCTGGGCTCACAGTACTTCCTCTGTGAACTGTT TGTGAATTTGCCTTCCCTTTGCCATCAGAGGGAAACTGTAAATCCTGTGTTTCATTACTTGAATGTAGTT ATTGAAATATATATTATATATATGCACATATATATAATAGGCTGTATATATTGCTCAGTATAGAAAGCAT GTAGGAGACTGGTGATGTGTTGACCTTTTTAAAAAAAAACATATGTATATGTGTGTNTATATATATACAC ACATATACATATATATGTGTATGTATGTACGTATGTATATATATATGACCAAAAGAAA
Notes:	The probe template was PCR amplified from IMAGE:421002 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:421002 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000773 same experiment
	EMAGE:21431 same embryo
	EMAGE:21427 same embryo
	EMAGE:21428 same embryo
	EMAGE:21432 same embryo
	EMAGE:21429 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source