EMAGE mouse gene expression spatial database

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EMAGE:21437

P4hb prolyl 4-hydroxylase, beta polypeptide ( MGI:97464)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000771. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000771_01	euxassay_000771_02	euxassay_000771_03	euxassay_000771_04

euxassay_000771_05	euxassay_000771_06	euxassay_000771_07	euxassay_000771_08	euxassay_000771_09

euxassay_000771_10	euxassay_000771_11	euxassay_000771_12	euxassay_000771_13	euxassay_000771_14

euxassay_000771_15	euxassay_000771_16	euxassay_000771_17	euxassay_000771_18	euxassay_000771_19

euxassay_000771_20	euxassay_000771_21	euxassay_000771_22	euxassay_000771_23	euxassay_000771_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21437_wholemount_strong.wlz

21437_wholemount_moderate.wlz

21437_wholemount_weak.wlz

21437_wholemount_possible.wlz

21437_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21437_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1010
Entity Detected:	P4hb, prolyl 4-hydroxylase, beta polypeptide ( MGI:97464)
Sequence:	sense strand is shown >T1010 TCCTCGAGCCTGTTGGCCTACTGGGCTCTCGTCGCCTTGGCTGTCCCGGCGGCGCCAACCCAACTGCCCC GCCCGCTGCAGACGTCCGACATGCTGAGCCGTGCTTTGCTGTGCCTAGCCCTGGCCTGGGCGGCTAGGGT GGGCGCCGACGCCCTGGAGGAGGAGGACAACGTCCTGGTGTTGAAGAAGAGCAACTTCGAGGAGGCGCTG GCGGCGCACAAGTACCTGCTGGTGGAGTTCTATGCCCCGTGGTGTGGCCACTGCAAAGCTCTGGCTCCCG AGTATGCCAAAGCTGCCGCAAAACTGAAGGCAGAAGGCTCTGAGATTCGACTAGCAAAGGTGGATGCCAC AGAAGAGTCTGACCTGGCTCAGCAGTATGGTGTCCGTGGCTACCCCACAATCAAGTTCTTCAAGAATGGA GACACAGCCTCCCCAAAGGAATATACAGCTGGCAGGGAAGCTGATGACATTGTCAACTGGCTGAAGAAAC GCACGGGCCCAGCAGCTACAACCCTGTCTGACACTGCAGCTGCAGAGTCCTTGGTGGACTCAAGCGAAGT GACGGTCATC
Notes:	The probe template was PCR amplified from IMAGE:2065055 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2065055 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000771 same experiment
	EMAGE:21434 same embryo
	EMAGE:21433 same embryo
	EMAGE:21436 same embryo
	EMAGE:21438 same embryo
	EMAGE:21435 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source