EMAGE mouse gene expression spatial database

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EMAGE:21438

Timp3 tissue inhibitor of metalloproteinase 3 ( MGI:98754)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000783_01	euxassay_000783_02	euxassay_000783_03	euxassay_000783_04	euxassay_000783_05

euxassay_000783_06	euxassay_000783_07	euxassay_000783_08	euxassay_000783_09	euxassay_000783_10

euxassay_000783_11	euxassay_000783_12	euxassay_000783_13	euxassay_000783_14	euxassay_000783_15

euxassay_000783_16	euxassay_000783_17	euxassay_000783_18	euxassay_000783_19	euxassay_000783_20

euxassay_000783_21	euxassay_000783_22	euxassay_000783_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21438_wholemount_strong.wlz

21438_wholemount_moderate.wlz

21438_wholemount_weak.wlz

21438_wholemount_possible.wlz

21438_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21438_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

visceral pericardium

strong

homogeneous

strong expression: see section 8 9 0 1 2 4 5 6 7 8 moderate expression: see section 7 3

medulla oblongata part of 4th ventricle choroid plexus

moderate

homogeneous

moderate expression: see section 0 2 4

diaphragm

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 6 7 8 9

limb

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 moderate expression: see section 1 2 3 4 5 8 9

head mesenchyme

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

trunk mesenchyme

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

vertebral axis musculature

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

vibrissa

strong

regional

strong expression: see section 3 4 moderate expression: see section 7 weak expression: see section 4 5 6 7

vibrissa epidermal component

strong

homogeneous

strong expression: see section 3 4 5 6 moderate expression: see section 7

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 0 1 2 3

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 0 1 2 3

thalamus ventricular layer

strong

graded

strong expression: see section 2 moderate expression: see section 0 1 weak expression: see section 3

lateral ventricle choroid plexus

moderate

homogeneous

moderate expression: see section 0

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 3

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 0 1 2 moderate expression: see section 0 1 2 3 weak expression: see section 9

pons mantle layer

strong

single cell

strong expression: see section 9 3 4

pons ventricular layer

strong

regional

strong expression: see section 8 9 0 1 2 6 moderate expression: see section 7 0 1 2 3 4 5 weak expression: see section 6 7 8 9 5 6 7

metencephalon part of 4th ventricle choroid plexus

moderate

homogeneous

moderate expression: see section 4 6 9 0

midbrain ventricular layer

strong

regional

strong expression: see section 9 0 moderate expression: see section 8 0 1 2 3 4 weak expression: see section 7 8 9 4 5

ventral grey horn

strong

single cell

strong expression: see section 9 0 1 2 3 5 weak expression: see section 9 0 1 2 3 4

spinal cord ventricular layer

strong

homogeneous

strong expression: see section 1 moderate expression: see section 2

nasal capsule

weak

regional

weak expression: see section 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

atrioventricular cushion tissue

moderate

homogeneous

moderate expression: see section 3 4

interventricular septum muscular part

strong

single cell

strong expression: see section 2 weak expression: see section 3

left ventricle cardiac muscle

strong

single cell

strong expression: see section 6 moderate expression: see section 5 7

right ventricle cardiac muscle

strong

single cell

strong expression: see section 7 8 9 1 moderate expression: see section 0

esophagus

strong

homogeneous

strong expression: see section 9 0 1 2

stomach

moderate

regional

moderate expression: see section 1 2 3 4 7 8

stomach pyloric region

strong

homogeneous

strong expression: see section 9

midgut

strong

regional

strong expression: see section 0

lower jaw incisor

moderate

regional

moderate expression: see section 1 2 weak expression: see section 6

lower jaw molar

moderate

regional

moderate expression: see section 1

upper jaw incisor

moderate

regional

moderate expression: see section 1 2 4

liver

strong

single cell

strong expression: see section 1 2 3 5 6 7 9 0 2 3 4 5 6 7 8 9

urinary system

strong

homogeneous

strong expression: see section 4 8 6 7 moderate expression: see section 5 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1014
Entity Detected:	Timp3, tissue inhibitor of metalloproteinase 3 ( MGI:98754)
Sequence:	sense strand is shown >T1014 CCTCGAGNCTGTTGGCCTACTGGAAAAAAAGAAAGAAACCCACATGCCATTTTTTTTTTTCTGATTCAAA TTCTACAGTAGAATGGAGGGCAGAATTACTGGGACACAATCACCACTCAGCTAGAATGGAGATTCAAGGA AATGGAAGGTACTAGAAACAGACTCCTCCAGCCTCCTCCCCATAGAATCCTAAGGGTGGGAAGAAGAAGA AGAAAAAAAAGCAAAAGCAAAACAAAACAGAAATCAAAAGTACACTCAAGTTCTTGTCCTGTATCAAATA TCCCTAGGTCATCTGCCACCCACTTTCCAAGACCTTTCTCCTACTCAACCAGGAAGTCATGGTTTCTGCA GTCCAGAGCCATGGCAACTGAGATTCTAAGCTCTCTTTGTCTCATCCAGGCAACTTTTCTGTCTCCCAAC CAAGGAATTCCTCTGCTCAGGCTCCTGTTGCCCAGGATAGCTCTCTTCTATAGCCAGGATGTGGAGCTTA AGGAGACTCCAATGTTTACTTTCTCCAGTCCTAGCTCTGCCATATAAACACTG
Notes:	The probe template was PCR amplified from IMAGE:2065144 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2065144 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000783 same experiment
	EMAGE:21434 same embryo
	EMAGE:21433 same embryo
	EMAGE:21437 same embryo
	EMAGE:21436 same embryo
	EMAGE:21435 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source