EMAGE mouse gene expression spatial database

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EMAGE:21441

Tuft1 tuftelin 1 ( MGI:109572)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000956_01	euxassay_000956_02	euxassay_000956_03	euxassay_000956_04	euxassay_000956_05

euxassay_000956_06	euxassay_000956_07	euxassay_000956_08	euxassay_000956_09	euxassay_000956_10

euxassay_000956_11	euxassay_000956_12	euxassay_000956_13	euxassay_000956_14	euxassay_000956_15

euxassay_000956_16	euxassay_000956_17	euxassay_000956_18	euxassay_000956_19	euxassay_000956_20

euxassay_000956_21	euxassay_000956_22	euxassay_000956_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21441_wholemount_strong.wlz

21441_wholemount_moderate.wlz

21441_wholemount_weak.wlz

21441_wholemount_possible.wlz

21441_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21441_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 7 8 9 2 3

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 3 4 7 8 weak expression: see section 0 1 2 9 0 1

lower jaw incisor

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9

lower jaw molar

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9

oral epithelium

moderate

regional

moderate expression: see section 8 9 0 1 2 3 4 5 6 7 8 9 1 2 3

laryngeal cartilage

weak

regional

weak expression: see section 3 4 5

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3901
Entity Detected:	Tuft1, tuftelin 1 ( MGI:109572)
Sequence:	sense strand is shown >T3901 CCACGCGTCCGGCGTGAAGATGAACGGGACGCGGAACTGGTGTACCTTGGTGGACGTGCACCCGGAGAGC CAGACCGCGGGCAGCGTGGACATTCTCAGGCTGACGCTCCAGAGTGAACTGACAGGAGATGAACTTGAGC ACATAGCCCAGAAGGCGGGCAGGAAGACCTATGCCATGATGCCTGGCCACTCCTCAGGTCATTCTCTGGC TTCAGAACTGGTGGAGTCCCATGATGGCCATGAAGAGATCATTAAGGTTTACTTGAAAGGGAGGTCTGGG GACAAGATGACCCATGAGAAGAACATCGACCAGCTGAAGAGTGAGGTCCAGTACATCCAGGAGGCCAGGA ACTGCCTGCAGAAGCTCCGGGAGGATATCAGTAGCAAGCTTGACAGGAGTCCAGGAGACCCTCTGCGTCA GCAGGAGATACAGGTGGTACTAGAAAAGCCAAATGGCTTTAGTCAGAGTCCCATGACTCTCTACAGTAGC CCACCTGAGGTGGACCCTAGCATGAGTGAAGATGTTGAGAGCTTGAAGAAGACTGTGCAGGAGTTGCTTG TCAAGCTGCGGGAGGCTGAGCGGCGACACCAGTCAGACCGTGTGGCCTTTGAGGTCACACTCAGCCGTTA TCAGCGAGAAGCAGAACAGAGTAATGTGGCCCTTCAGAGAGAGGAGGACAGAGTGGAGCAGAAAGCGGCA GAAATCGAGGAGCTACAGAGGCGCTTGCTGGGGATGGAGGCGGAGCATCAGGCCTTACTGGTGAAAGTCA GGGAAGGAGAGATGGCCCTAGAAGAGCTTCGGATCAAGAACGCTGACTGTCAAACAGAAAGAGAAAAGTC TGCTGCTCTAGAAAAGGAAGTGGCTGGGTTTCGGGAGAAGATCCACCACTTGGATGACATGCTGAAGAGC CAGCAGCGGAAAGTTCGGCAGATGATAGAGCAGCTCCAGAATTCAAAAGCTGTGATCCAGTCAAAGGATG CCACCATTCAAGAGCTCAAGGAGAAAATTGCCTATCTGGAGGCTGAGAACTTAGAGATGCACGACCGCAT GGAGCATCTAATAGAGAAGCAGGTCAGCCACGGAAACTTCAGCACACAGGCCCGTGCCAAGACAGAGAAC CTGGGCAGTGTCAGGATATCGAAGCCCCCCAGCCCCAAGCCTATGCCTCTCATCCGGGTGGTGGAAACCT GAGCTGCCAGGAGATGGACGTTGCCGTTGCAGTTGCCTCAGTCCTGCAGAGAAGCCCTCCTCCCTTGTAG GCGGTTGATACTGACTGACTTCCTGACTACCCTGGTACCCGCAGGTCCTGGGCTCTTGCCCCAGGCTCCT GTCCTCTGCTTGCTGGCAGATGGGTCCGGAGCGCCTGTCTCCTCAGGACACTGCAGACCTTGGGAAGACA GTCTCCTGCTAGCAGGAACGAAGCAATACCCTTATTCTCAGTTAGTGTTTTCTCCACATAACAGAGCTTC CCTGCTGTGTGCACTGGAATCTAGCTGCCCAAAAGCCAGGCCCTTGGTAAGTCCAGAGAAGTGACAAAAT TTGTCTCAGCCCAAAAAGCTAGAAGCCCAGATGCGCAGAGTGACTGGAGCGTGTCCTGGGGGAGTGACGT TCCTTCTGCAAACACAGCTCAGTTCTTAGCAACAAACTGTTTGTTTTCCGCTTGACTCCACCCTTGTGCC ATGCTGACCAGGGCCTTTTCCAGACAGCCAATGGGGGATATAGTTGGCCAGGCCTGGTCTTAGCCAGGGA ACCTGGCCTACTAGATAGCCTTGCCTTTTGGGGACACAGGAATGTCCATTCAGGCTGCAGTTTTGGGTAG GAAGATTCTCTGGGGCTATGAATAGTTCATGGAAGCTCCCCGTCTGTGGGTTTTCCCCTGCGGTGTCCTG AGAGGCACACCTTAGCCTCCCTAACCCAGAACTGAGAACTGTCTCCTCTGTCAGTCTCAGCAAAACTGCT ACTGTATTTATTTTGCTAAGTTATTGCTGGTTTTGCTTATATCTCATAATTGATTTAGTACCAGTGTTCT GCAGCTTTCTGCCGAGGTATTTAGGTTTACCTGGACATGGGGAAAGTATCCTGGTTGGCCTCGTTGGTTT CACAGTGTCATACTAACTCCCCATGCCATCCCTCTAGCTGCTGCTTTTCCTTTCTTTCCCTAGAGTCTAC CTGTAACTCAGGAGGCTCATGTCTGTCCCAGAGTGTGTGTTGTCTGGGGAATAGGGCAGTGGGGACTGTT TGGGAGGAAACAGAGGTTCTTCTAATCTCTCTGAACACTTCCACCCATAACAACCTCTATCCCAGCCCTG AATGACCCTGCAGCTCATCGTGTTCGCTATAGTGTGGTCTAGTAGCCTCATGAGTGTTCTACTTGGCTCA CAACCAGAAGCCTTAAGTCTTGTGTGTTTCTGTGTCAGGACTAGACCCAGATCCTGGCTTTGTCTGTACA GTGTCCTGAGACTTCAGGCAGAACGAGTGGGTTGTACATAATCTCCGTGCCCCTTCAAATTCTTTTCCTG TCTGTGACATAGTCTTTGTATGGCAACTTTAATAAATTATGCTAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5064658 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5064658 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000956 same experiment
	EMAGE:21443 same embryo
	EMAGE:21439 same embryo
	EMAGE:21440 same embryo
	EMAGE:21442 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source