EMAGE mouse gene expression spatial database

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EMAGE:21449

Bcap29 B-cell receptor-associated protein 29 ( MGI:101917)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000840_01	euxassay_000840_02	euxassay_000840_03	euxassay_000840_04	euxassay_000840_05

euxassay_000840_06	euxassay_000840_07	euxassay_000840_08	euxassay_000840_09	euxassay_000840_10

euxassay_000840_11	euxassay_000840_12	euxassay_000840_13	euxassay_000840_14	euxassay_000840_15

euxassay_000840_16	euxassay_000840_17	euxassay_000840_18	euxassay_000840_19	euxassay_000840_20

euxassay_000840_21	euxassay_000840_22	euxassay_000840_23	euxassay_000840_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21449_wholemount_strong.wlz

21449_wholemount_moderate.wlz

21449_wholemount_weak.wlz

21449_wholemount_possible.wlz

21449_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21449_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 3 5 6 weak expression: see section 1 2 4

submandibular gland primordium

weak

regional

weak expression: see section 7 8 9 0 9 0 1 not examined expression: see section 1

vibrissa

weak

regional

weak expression: see section 6 7 8 9 2 3

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 1 2 3 4 6 7 8 9 0 1 2 6 7 8 9 0 1 2 3

vagus x ganglion

moderate

homogeneous

moderate expression: see section 9 7 8 weak expression: see section 8

dorsal root ganglion

weak

regional

weak expression: see section 8 9 7

liver lobe

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 7 8 9 0 1 2 weak expression: see section 4 5 6 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T183
Entity Detected:	Bcap29, B-cell receptor-associated protein 29 ( MGI:101917)
Sequence:	sense strand is shown >T183 TGACGGGCGGCGGGCGGCCCCGCTGCTGACCTCGCGTCCGGCGGCTCGGGCCGCGGCGTTCTGCGGCCCG AGCCACCTCGGGACCGGGGAAGAACCTGCGGGGCCGTCCGGCGCACTGCCTCGTCACCCGGAGTTAGGCG AACTCCCGGCAGGCCCCACGCCGCGTGTCTGGCCGTTCACCCATGTTTCTCTAAAATTGAAATCTTTCTC TGTATAGGTGTGAAGAAAAAACAAAACAAAACGATGACAATCCAATGGGCGGCGGTTGCCAGCTTTCTTT ATGCTGAAATAGGACTCATTTTACTCTTCTGTCTGCCCTTTATTCCTCCACAGAGATGGCAGAAGATTTT TTCATTTAGTGTCTGGGGTAAGATTGCAAGTTTTTGGAACAAAGCTTTTCTTACCATTATAATACTATTG ATCATTCTGTTTCTAGATGCCGTGAGAGAAGTAAGAAAGTATTCGTCCACGAATGTTGTAGAGAAGAA
Notes:	The probe template was PCR amplified from IMAGE:2646883 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2646883 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000840 same experiment
	EMAGE:21445 same embryo
	EMAGE:21447 same embryo
	EMAGE:21446 same embryo
	EMAGE:21448 same embryo
	EMAGE:21444 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source