EMAGE mouse gene expression spatial database

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EMAGE:21457

Cnn1 calponin 1 ( MGI:104979)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000846_01	euxassay_000846_02	euxassay_000846_03	euxassay_000846_04	euxassay_000846_05

euxassay_000846_06	euxassay_000846_07	euxassay_000846_08	euxassay_000846_09	euxassay_000846_10

euxassay_000846_11	euxassay_000846_12	euxassay_000846_13	euxassay_000846_14	euxassay_000846_15

euxassay_000846_16	euxassay_000846_17	euxassay_000846_18	euxassay_000846_19	euxassay_000846_20

euxassay_000846_21	euxassay_000846_22	euxassay_000846_23	euxassay_000846_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21457_wholemount_strong.wlz

21457_wholemount_moderate.wlz

21457_wholemount_weak.wlz

21457_wholemount_possible.wlz

21457_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21457_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

strong

regional

strong expression: see section 3 4 5 6 7 8 9 0

not examined

homogeneous

not examined expression: see section 3 4

not examined

regional

not examined expression: see section 3

esophagus

strong

regional

strong expression: see section 5 6

stomach

strong

regional

strong expression: see section 2 3 4 5 6 7 8 9 0 1 not examined expression: see section 2 6 7 8 9 0 1 2

hindgut

strong

regional

strong expression: see section 3 4 5 6 7

midgut

strong

regional

strong expression: see section 8 9 0 1 2 3 4 5 6 7 8 9 0 1

not examined

regional

not examined expression: see section 2 3 4 5

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2307
Entity Detected:	Cnn1, calponin 1 ( MGI:104979)
Sequence:	sense strand is shown >T2307 GGCCTCGAGGCCAGATTCGTCGACATGGTCCCCAACAGTGCCCGAAGGAAGCACTGAGTCGAGCTGTTTC AGCTGCCCCCCTTTATTCCCTCACAAGTACCTCCCAGAACCAGAAACCCTTCCAGCGAAGCCCCCAGAAC CCAGGCTTGGCCTGACCCCTGCCCCATTCCTGCGACGGGAGCAGATGCATGCCTGGAAACGCAGTGGACA CACGCATTTTGGTTTTGCAGTGACTGGTGTTTGTGGATGTGACAGCAGCGTTTGTAACTCGAACACATTC TTTTCTATTTCACTGGAGCACAATAAATGGCTGTGACATCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1138605 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1138605 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000846 same experiment
	EMAGE:21458 same embryo
	EMAGE:21456 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source