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EMAGE:21458

Exosc10 exosome component 10 ( MGI:1355322)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21458 EMAGE:21458 EMAGE:21458 EMAGE:21458 EMAGE:21458
euxassay_000850_01 euxassay_000850_02 euxassay_000850_03 euxassay_000850_04 euxassay_000850_05
EMAGE:21458 EMAGE:21458 EMAGE:21458 EMAGE:21458 EMAGE:21458
euxassay_000850_06 euxassay_000850_07 euxassay_000850_08 euxassay_000850_09 euxassay_000850_10
EMAGE:21458 EMAGE:21458 EMAGE:21458 EMAGE:21458 EMAGE:21458
euxassay_000850_11 euxassay_000850_12 euxassay_000850_13 euxassay_000850_14 euxassay_000850_15
EMAGE:21458 EMAGE:21458 EMAGE:21458 EMAGE:21458 EMAGE:21458
euxassay_000850_16 euxassay_000850_17 euxassay_000850_18 euxassay_000850_19 euxassay_000850_20
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euxassay_000850_21 euxassay_000850_22 euxassay_000850_23 euxassay_000850_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21458Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21458_wholemount_strong.wlz
21458_wholemount_moderate.wlz
21458_wholemount_weak.wlz
21458_wholemount_possible.wlz
21458_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21458_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1000
Entity Detected:Exosc10, exosome component 10 ( MGI:1355322)
Sequence:sense strand is shown

>T1000
TCTCGAGNCTGTTGGCCTACTGGAACATCTCGCGAGAAGAGCCGCCCTAGGACGGAAAAATGGCGCCTCC
CAGTCCCCGGGAGCATCAGTCCGCGCCGGCGACCAGTGCGACCAAGCCTGACGCGGAGATGGTACTGCCT
GGCTTCCCCGATGCAGACAGCTTCGTAAAGTTTGCACTTGGGTCGGTAGTGGCAGTTACCAAGGCATCCG
GGGGCCTGCCACAGTTCGGTGACGAGTATGATTTTTACAGAAGTTTCCCTGCCTTCCAGGCATTCTGTGA
GACACAAGGAGACAGGTTACTGCAGTGCATGAGTCGGGTAATGCAGTACCATGGCTGTCGCAGCAACATC
AAAGACCGAAGTAAAGTGACTGAATTGGAGGACAAGTTTGATTTATTAGTCGATACCAATGACGTGATAT
TGGAGAGAGTGGGCATGTTACTGGATGAAGCCTCCGGTGTGAACAAGCATCAGCAGCCTGTCCTTCCTGC
AGGGCTGCAGGTCCCCAAAACAATAGTATCCAGCTGGAATCGGAAGGCAGGAGAGTATG
Notes:The probe template was PCR amplified from IMAGE:1973164 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1973164 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000850 same experiment
 EMAGE:21457 same embryo
 EMAGE:21456 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS