EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21473

Arl5a ADP-ribosylation factor-like 5A ( MGI:1922673)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000977. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000977_01	euxassay_000977_02	euxassay_000977_03	euxassay_000977_04

euxassay_000977_05	euxassay_000977_06	euxassay_000977_07	euxassay_000977_08	euxassay_000977_09

euxassay_000977_10	euxassay_000977_11	euxassay_000977_12	euxassay_000977_13	euxassay_000977_14

euxassay_000977_15	euxassay_000977_16	euxassay_000977_17	euxassay_000977_18	euxassay_000977_19

euxassay_000977_20	euxassay_000977_21	euxassay_000977_22	euxassay_000977_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21473_wholemount_strong.wlz

21473_wholemount_moderate.wlz

21473_wholemount_weak.wlz

21473_wholemount_possible.wlz

21473_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21473_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate

weak

homogeneous

weak expression: see section 6 7 3 4

facial vii ganglion

weak

homogeneous

weak expression: see section 2 3 4 8 9

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 5 weak expression: see section 4 6 7

superior glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 5 6 7

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 5 weak expression: see section 1 2 3 4 6 6 7 8 9 0

not examined

homogeneous

not examined expression: see section 5

superior vagus x ganglion

weak

homogeneous

weak expression: see section 6 not examined expression: see section 5

vestibulocochlear viii ganglion cochlear component

moderate

homogeneous

moderate expression: see section 5 6 weak expression: see section 4 7

vestibulocochlear viii ganglion vestibular component

moderate

homogeneous

moderate expression: see section 5 6 weak expression: see section 4 7

liver lobe

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 2 3 4 5 6 7 8 0 1 2 weak expression: see section 9 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1579
Entity Detected:	Arl5a, ADP-ribosylation factor-like 5A ( MGI:1922673)
Sequence:	sense strand is shown >T1579 CAGAAATCTCCCAGTTTTTGAAGCTTACTTCTATAAAAGACCACCAGTGGCATATTCAAGCATGTTGCGC TCTTACTGGCGAGGGGTTATGCCAAGGACTTGAATGGATGATGTCACGGCTGAAGATCAGATGATCTCTT CTGACCCCTCCTCACAGACTCTGTATAAAGAAAGTGCTGGACTTTTCCTGAAAGCTGCAAAAAAATGGTT TAGATATATTTATAATAAACTGACTTAAGAGTTTTCTATAAGAAGAAAATTAAGACCACTTATTTGAAAA CAAAGATGAAATGTCACCACCCGTTTGCGCTCTCATTCCTTCCAAGCCTGTGACTGGTATTTTCTCATGA TGAATCCTCTCAGGACATGGCAGAGCTTGGCGAGTACAAAGGGAGAGGAAGACATTTGAACTTTAGAACT TTGTTTCAGCGTGACTCTCCCACCCTGCCCCAAGTTGAATGTTCCCTTCACATTGAATCAAATACAAATC AGCACAGATACTGTTTCCAGTTTTTAAAAA
Notes:	The probe template was PCR amplified from IMAGE:934823 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:934823 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000977 same experiment
	EMAGE:21472 same embryo
	EMAGE:21471 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source