EMAGE mouse gene expression spatial database

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EMAGE:21476

Ube4a ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) ( MGI:2154580)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000890_01	euxassay_000890_02	euxassay_000890_03	euxassay_000890_04	euxassay_000890_05

euxassay_000890_06	euxassay_000890_07	euxassay_000890_08	euxassay_000890_09	euxassay_000890_10

euxassay_000890_11	euxassay_000890_12	euxassay_000890_13	euxassay_000890_14	euxassay_000890_15

euxassay_000890_16	euxassay_000890_17	euxassay_000890_18	euxassay_000890_19	euxassay_000890_20

euxassay_000890_21	euxassay_000890_22	euxassay_000890_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21476_wholemount_strong.wlz

21476_wholemount_moderate.wlz

21476_wholemount_weak.wlz

21476_wholemount_possible.wlz

21476_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21476_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa epidermal component

moderate

regional

moderate expression: see section 9 weak expression: see section 3 4 5 8 0

not examined

homogeneous

not examined expression: see section 4 5

medulla oblongata basal plate

moderate

regional

moderate expression: see section 5 7 8 2 4 5 weak expression: see section 6 6

medulla oblongata basal plate mantle layer

strong

homogeneous

strong expression: see section 9

trigeminal v ganglion

strong

homogeneous

strong expression: see section 1 2 3 6 7 5 6 7 8 9 0 1 moderate expression: see section 4 5

vagus x ganglion

strong

homogeneous

strong expression: see section 5 6 7 7 moderate expression: see section 5 6

ventral grey horn

strong

homogeneous

strong expression: see section 9

dorsal root ganglion

strong

regional

strong expression: see section 9 0 5 6 7 moderate expression: see section 6 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T979
Entity Detected:	Ube4a, ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) ( MGI:2154580)
Sequence:	sense strand is shown >T979 TCCTCGAGNCTGTTGGCCTACTGGACCTTTTGGTCGCTGTAGCTGTGAGGTGTCGTCGTCCCTGCCCTTT CAGGCTCCGGTACTTTTATTTTCATGTGTAAGCTCAATGACTAAGTCTAACCTGGATCTCCACCGAGGTT CTCAACAGGTGATCTGTGGGCCCTCATTTTTTCCTCCGCTCCCCTAGGGCTGGATGGAGGACACCTTAAA GTGAAATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCT GGCTGATGCCAAGCAGTTTGCAGCAATCCAGAAAGAGCAGCTGAAGCAGCAGTCCGGTAGTGTACTTGGT TCTGAGGAAGAAGCGTTGTGTAACAGCTTACATGTTCCAGCAGATGAGCTCCCAGCAAGCCCAGATGACT CGGATAATAGTGTGTCAGAGAGCCTGGATGAGTTTGACTACTCTGTATCTGAGATTAGCCGCTCCTTCCG GACACGCCAAGAAATGTGTGAGCAACTCAACATCAATCACATGATCCAGAGGATC
Notes:	The probe template was PCR amplified from IMAGE:1972320 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972320 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000890 same experiment
	EMAGE:21477 same embryo
	EMAGE:21479 same embryo
	EMAGE:21478 same embryo
	EMAGE:21475 same embryo
	EMAGE:21474 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source