EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21477

Atp6v1c1 ATPase, H+ transporting, lysosomal V1 subunit C1 ( MGI:1913585)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000870. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000870_01	euxassay_000870_02	euxassay_000870_03	euxassay_000870_04

euxassay_000870_05	euxassay_000870_06	euxassay_000870_07	euxassay_000870_08	euxassay_000870_09

euxassay_000870_10	euxassay_000870_11	euxassay_000870_12	euxassay_000870_13	euxassay_000870_14

euxassay_000870_15	euxassay_000870_16	euxassay_000870_17

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21477_wholemount_strong.wlz

21477_wholemount_moderate.wlz

21477_wholemount_weak.wlz

21477_wholemount_possible.wlz

21477_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21477_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 3 4 3 4 5

olfactory cortex marginal layer

moderate

homogeneous

moderate expression: see section 6 7 8 9 0

medulla oblongata alar plate mantle layer

weak

regional

weak expression: see section 8 9

medulla oblongata basal plate

moderate

homogeneous

moderate expression: see section 5 6 0 1

facial vii ganglion

moderate

homogeneous

moderate expression: see section 2 4 2 3 4 5

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 4 2 3

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 4 2

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 1 2 3 4 5 6

vagus x ganglion

moderate

homogeneous

moderate expression: see section 3

inferior vagus x ganglion

moderate

homogeneous

moderate expression: see section 6 1

superior vagus x ganglion

moderate

homogeneous

moderate expression: see section 5

vestibulocochlear viii ganglion vestibular component

moderate

homogeneous

moderate expression: see section 2 3 3 4

ventral grey horn

moderate

regional

moderate expression: see section 7 8 9 0

dorsal root ganglion

moderate

regional

moderate expression: see section 5 6 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T980
Entity Detected:	Atp6v1c1, ATPase, H+ transporting, lysosomal V1 subunit C1 ( MGI:1913585)
Sequence:	sense strand is shown >T980 TCGAGNCTGTTGGCCTACTGGACTTGCTCTGGGCGCAGGGGTCGAGGAAGCCGAAAGGCCGGAGCTTAGG TCCGGGAGGGATGGAGCGCTGAGCGGTTAACGTCTACCAGGCTTCCTGCCTCCGCACCTGTCGCCGCCGC CGCCTCCTGAGACACCTCGGCTTCTCCCTTGCTTGCCAAAGAGGTAACAAACATGACTGAGTTCTGGCTC ATATCTGCTCCTGGGGAGAAAACCTGTCAGCAAACATGGGAGAAACTACATGCAGCGACCACCAAGAACA ATAATCTTGCCGTCTCTTCCAAGTTCAACATTCCTGACCTAAAGGTTGGCACGTTGGATGTCTTGGTTGG CTTGTCGGATGAACTGGCTAAACTGGATGCATTTGTAGAAGGTGTGGTCAAGAAAGTGGCTCAGTACATG GCTGATGTGCTGGAGGACAGCAAAGATAAAGTTCAGGAGAACTTGCTGGCCAGCGGAGTTGACTTGGTTA CTTACATAACAAGGTTCCAGTGGGATATGGCTAAATATCCAATCAAGCAGTCTCTGAAAAATATTT
Notes:	The probe template was PCR amplified from IMAGE:1972366 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972366 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000870 same experiment
	EMAGE:21479 same embryo
	EMAGE:21476 same embryo
	EMAGE:21478 same embryo
	EMAGE:21475 same embryo
	EMAGE:21474 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source