EMAGE mouse gene expression spatial database

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EMAGE:21509

Gjd2 gap junction protein, delta 2 ( MGI:1334209)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001245. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001245_01	euxassay_001245_02	euxassay_001245_03	euxassay_001245_04

euxassay_001245_05	euxassay_001245_06	euxassay_001245_07	euxassay_001245_08	euxassay_001245_09

euxassay_001245_10	euxassay_001245_11	euxassay_001245_12	euxassay_001245_13	euxassay_001245_14

euxassay_001245_15	euxassay_001245_16	euxassay_001245_17	euxassay_001245_18	euxassay_001245_19

euxassay_001245_20	euxassay_001245_21	euxassay_001245_22	euxassay_001245_23	euxassay_001245_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21509_wholemount_strong.wlz

21509_wholemount_moderate.wlz

21509_wholemount_weak.wlz

21509_wholemount_possible.wlz

21509_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21509_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 7 8 9 0 1 2 3 4 5 6

telencephalon mantle layer

weak

regional

weak expression: see section 2 3 4 5 6 7 8 1 2 3 4 5 6 9 0 1 2 3 4

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 0 1 2 3 5 6 7 8 9

metencephalon rest of alar plate mantle layer

weak

regional

weak expression: see section 4 5 6 7 8 9 0 1 2 5 6 8 9 0 1 2

pons mantle layer

weak

regional

weak expression: see section 5 6 8 9 0

ventral grey horn

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7

intermediate grey horn

moderate

regional

moderate expression: see section 4

cervico-thoracic ganglion

strong

regional

strong expression: see section 1 2 7

cervical ganglion

strong

regional

strong expression: see section 0 8

thoracic ganglion

strong

regional

strong expression: see section 1 2 3 4 5 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5389
Entity Detected:	Gjd2, gap junction protein, delta 2 ( MGI:1334209)
Sequence:	sense strand is shown >T5389 AGCCCCGCAGCTAGCTCTGCGCGCCGCAGCGCGGGAGCGCCAGGTGCCCGCTCCAGTGACAGGCGCCGGG GTCCGTGCGCGGACCCTCCCTCAGACCGCAAGATCGCGGGGACCGCGGCGCAGGCACCGGTCTCCCGCGG GCAGGAGCTCGGCCACCTGCACGCTGCCACCCAGCCCCGCCCCTCGCCGGGAGACTGCGGGAGTCCATCT GACCCCCGGACGGCACCGAGGTGCTGTCCAGCTCTTCCGCACCGCGCCAGCAGCTCCATTGGTTGGGACG CACATAGATTGCTTCCCCCACTCCCCTCTTTTAAGTGCAATAAAGGGGGAGGGCCTCGTGGATTTTTTTA AAAAACAATTTTTGTTTGCTTCTTTAATTGGGGGCAATTCGCTCCCTGTCAGGAGGTGAGGCCCGGGGAG GGGCCAGGACCAAGAACGTGCCCGGTACTGCCCAGTCTTTGTCTGCTGCCTCCGGATGCACAGCGATGGG GGAATGGACCATCTTGGAGAGGCTGCTGGAAGCCGCGGTGCAGCAGCACTCCACTATGATTGGGAGGATC CTGTTGACTGTGGTGGTGATCTTCCGGATACTCATTGTGGCCATTGTAGGGGAGACGGTGTACGATGATG AGCAGACCATGTTTGTGTGCAACACCCTACAGCCCGGCTGTAACCAGGCCTGCTATGACCGCGCCTTTCC CATCTCCCATATACGTTACTGGGTCTTCCAGATCATAATGGTGTGCACCCCCAGTCTCTGTTTTATCACC TATTCTGTGCACCAATCCGCCAAGCAGCGAGAACGCCGGTACTCTACTGTCTTCCTAGCCCTGGACAGAG ACCCTGCTGAGTCTATAGGGGGACCTGGAGGAACTGGGGGTGGGGGCAGCGGAGGGAGCAAACGAGAAGA TAAGAAGTTGCAAAATGCCATTGTCAATGGGGTGCTGCAGAACACAGAGACCACCAGTAAGGAGACAGAA CCAGATTGCTTAGAGGTTAAAGAGCTGACTCCACATCCATCTGGGCTGCGCACAGCAGCAAGGTCCAAGC TCCGAAGACAGGAAGGTATCTCCCGCTTCTACATCATCCAAGTGGTGTTTCGAAATGCTCTGGAGATTGG GTTTCTGGTGGGCCAGTACTTTCTATATGGCTTCAGTGTTCCAGGGTTGTATGAGTGCAACCGTTACCCC TGCATCAAGGAGGTAGAATGTTATGTGTCTAGACCTACCGAGAAGACAGTCTTTCTGGTGTTCATGTTTG CTGTGAGCGGCATTTGTGTGGTGCTCAATCTGGCTGAACTTAACCATCTGGGATGGCGGAAGATCAAACT GGCTGTCCGGGGAGCCCAGGCCAAGAGGAAGTCAGTCTATGAGATACGTAACAAAGATCTGCCTCGAGTC AGTGTTCCCAATTTCGGCAGGACTCAGTCCAGTGACTCTGCCTATGTGTGAAAGGGCAGGTTTGGGGAAG GCCTGCTAGGTGGCAAGTAGCCCCAAGGCAGATAATGACTCGGGGTTTACAGGTTTGCCCTCATTCGGGT GATGCTCGCTGTGAAGGTCTAGGATACAGATATTAAGAGGTCTTCATCAACAAAGAGGAGAGGTAGAACT GTCATCACGTGTTACATCATCAGTTCTACAGAAACCATTGGGTAGAGTGACAAGATGGCTAAGCTGGACA TTCTTGGGCCTTCTTCTTTTAATGGCCCTTCCTGCTCCTGATCATAGTGAACTGGCATAGCTGCCAAGCA AGAATTAGCTCTGCTGAGCTCTGTATTCTGGTGAATGGCATGAGTCAAACACTCATTGATATAAAATTTG TGACCCATCTCAGCACATACCCTTATCCTGGGCTGCAGAAAAAAAAATCCTGGAGGATATTTCTCCCCCT TCAGCATGCCCCTTTCCCAAACCCAGGATAGCATGCCAGCTTTTCTTTTTCCTTGATCTGGCCTTACATT AGCCCTAACACAGTTTATCTGCAAGCTAGAGGGAATTACGTGGGGTGCTTTTTGGAGACTAGCCAGGGCA GAGGTTTGCAGTAGAGCCCCATATGACTGGCTCTTAGTCATTAAACAAAACACAAACCTTAGGAATGTCT CAATATTTCCAATTTTTTAAAACTCAAATTGCACTGGTGCTGTTTATTAGGGGTGGGGAATGGGAGGGGG GAATTTCAACACCACTTATGAGATGGTCATTTCACTCACCGTTGAGCATGTGTTTATGAGCTTTGGGATA TTTCTGCCTTCTAGATTATGGACTGTGGATAGAATTAAAAATATGTGGGTTTTTGTTTTGTTTTGTTGTT ATTGTTGTTGTTGTTGAAATGGAGGGTATCTACTCAAGCCACAGAATTTCTAACTGGTAGATCTTACTAT AATTTCTTACCTAATCTTGTGCTACGCAGCTGCACATCATTTTGCCATGGTTTGAACTTACCTGTGTGCT CACACCAGAGACCTTAATCAGCCCGTGTCAATCCCAACTTATTGTGTCATCAATTATTTTTTGCAATACA CACACATGCACACAATTACTGGCTTAACAAACCTGGAAGTAAATATTATTGGTTAAGATATGTTTATGGA TATTTATTTGAGACATATACCAAAGGTTCATAAACCTGTTGTTTGGACTGAGACAATTTTCCTCTCGTTG CTCTTATTCATTTAAGTGCCATATTCCTCCTTTCCTGCCCACTGTGCATTTAGCTGAAGCACTAGGCAAG AGTAGCATTGAGATAATATGTTTTTATGGAAGCCCTCTATTTCATTTGAATCCTTCTTCTTATGGGTTTG TTTTTGATTTAGATATGGATGTGGTATGTTATATTTGGATTTGTTTAAAATAAAGTTCTTGCCTTTTTTT GTAAACTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364942 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364942 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001245 same experiment
	EMAGE:21512 same embryo
	EMAGE:21507 same embryo
	EMAGE:21511 same embryo
	EMAGE:21510 same embryo
	EMAGE:21508 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source