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EMAGE:21511

Syt1 synaptotagmin I ( MGI:99667)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511
euxassay_001257_01 euxassay_001257_02 euxassay_001257_03 euxassay_001257_04 euxassay_001257_05
EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511
euxassay_001257_06 euxassay_001257_07 euxassay_001257_08 euxassay_001257_09 euxassay_001257_10
EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511
euxassay_001257_11 euxassay_001257_12 euxassay_001257_13 euxassay_001257_14 euxassay_001257_15
EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511
euxassay_001257_16 euxassay_001257_17 euxassay_001257_18 euxassay_001257_19 euxassay_001257_20
EMAGE:21511 EMAGE:21511 EMAGE:21511 EMAGE:21511
euxassay_001257_21 euxassay_001257_22 euxassay_001257_23 euxassay_001257_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21511Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21511_wholemount_strong.wlz
21511_wholemount_moderate.wlz
21511_wholemount_weak.wlz
21511_wholemount_possible.wlz
21511_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21511_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
foot mesenchyme
weak weak
regionalweak expression: see section 2 3 4 0 2 3
vertebral axis musculature
weak weak
regionalweak expression: see section 1 2 3 4 5 6 7 8 2 3 4 5 6 0 1 2 3 4
pectoralis major
moderate moderate
regionalmoderate expression: see section 7 8 9 0 3 4 5 weak expression: see section 6 1 2 6 7 8 9 0 1 2 3
pectoralis minor
moderate moderate
regionalmoderate expression: see section 7 8 9 0 3 4 5 weak expression: see section 6 1 2 6 7 8 9 0 1 2 3
adrenal gland
moderate moderate
regionalmoderate expression: see section 7 8 9 0 5 6 7 8
pituitary gland
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 6
hypothalamus mantle layer
strong strong
homogeneousstrong expression: see section 8 9 0 1 2 3 4 5 6 7 8
diencephalon lateral wall mantle layer
strong strong
homogeneousstrong expression: see section 7 8 9 0 1 2 3 4 5 6 7 8 9
not examined not examined
homogeneousnot examined expression: see section 9
telencephalon mantle layer
strong strong
homogeneousstrong expression: see section 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4
telencephalon marginal layer
strong strong
homogeneousstrong expression: see section 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4
olfactory cortex marginal layer
strong strong
homogeneousstrong expression: see section 0 1 2 3 5 6 7 8
medulla oblongata alar plate mantle layer
strong strong
homogeneousstrong expression: see section 7 8 9 0 1 4 5 6 8 9 not examined expression: see section 2 3
medulla oblongata basal plate mantle layer
strong strong
homogeneousstrong expression: see section 2 3 4
metencephalon rest of alar plate mantle layer
strong strong
homogeneousstrong expression: see section 3 4 5 6 7 8 9 0 7 8 9 0 1 2 not examined expression: see section 1 2
pons mantle layer
strong strong
homogeneousstrong expression: see section 1 2 3 4 5 6 7 8 9
midbrain mantle layer
strong strong
homogeneousstrong expression: see section 5 6 7 8 9 0 1 2 4 5 6 7 8 9 0
midbrain marginal layer
strong strong
homogeneousstrong expression: see section 0 1 2 3
facial vii ganglion
strong strong
regionalstrong expression: see section 4 5 6 7 1 2
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 7 8 9 0
trigeminal v ganglion
strong strong
regionalstrong expression: see section 3 4 5 6 7 8 9 8 9 0 1 2 3
vagus x ganglion
strong strong
regionalstrong expression: see section 9 8
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 7 weak expression: see section 8 8 9 0
spinal cord mantle layer
strong strong
homogeneousstrong expression: see section 9 0 1 2 3 4 5 6 7
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 1 6 7
cervical ganglion
strong strong
regionalstrong expression: see section 0 7
thoracic ganglion
strong strong
regionalstrong expression: see section 2 3 5
dorsal root ganglion
strong strong
regionalstrong expression: see section 8 9 0 1 5 6 7 8 9 1
cornea stroma
strong strong
homogeneousstrong expression: see section 1 2 3 4
retina
moderate moderate
regionalmoderate expression: see section 1 2 3 4 3 4
anterior naris epithelium
moderate moderate
regionalmoderate expression: see section 3 6
external naris
moderate moderate
regionalmoderate expression: see section 2
external naris epithelium
moderate moderate
regionalmoderate expression: see section 7 weak expression: see section 8
posterior naris epithelium
moderate moderate
regionalmoderate expression: see section 5
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 0 1 2 3 5 6 7 8 weak expression: see section 8 9 9
esophagus
weak weak
regionalweak expression: see section 2 3 4
tongue
weak weak
regionalweak expression: see section 1 2 3 5 6
stomach
moderate moderate
regionalmoderate expression: see section 2
stomach fundus epithelium
moderate moderate
regionalmoderate expression: see section 2 3 4 5 6 7 8
foregut-midgut junction epithelium
moderate moderate
regionalmoderate expression: see section 9 0 1 2 3 4
rectum epithelium
moderate moderate
regionalmoderate expression: see section 1
duodenum caudal part epithelium
moderate moderate
regionalmoderate expression: see section 0 1 2 3 4 5 6
midgut loop epithelium
moderate moderate
regionalmoderate expression: see section 6 7 8 0 1 2 3 4 5 6
rest of midgut epithelium
moderate moderate
regionalmoderate expression: see section 1
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 1 2 6 8
oral epithelium
moderate moderate
regionalmoderate expression: see section 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 1
ureter
moderate moderate
regionalmoderate expression: see section 9 0 1 5 6 7
urethra of male
strong strong
regionalstrong expression: see section 1 5
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5393
Entity Detected:Syt1, synaptotagmin I ( MGI:99667)
Sequence:sense strand is shown

>T5393
GGGGACCAAGACTCGCACCATCTCCCGGTCGGTCCTCGCTCCAGGAAAAGAAGACTTTGGCGCGCTCGAA
CAACCAACATCCGCAGTCTGATCAGAAGACATTGCCCTTGCCGTCCCGAGAGCTCCAGCAGAACATCTCG
TTAAGATTGAAGAAAGGAGATTCCAAAAGGAACAAAAAAACCCAAATACTCCAGACTACCCCAGCAGAAC
ATTCCGCTTGAACCAAAAATGGTGAGTGCCAGTCGTCCTGAGGCCCTGGCTGCCCCTGTCACCACTGTTG
CGACCCTTGTCCCACACAACGCCACTGAGCCAGCCAGTCCTGGGGAAGGGAAGGAAGATGCCTTTTCCAA
GCTGAAGCAGAAGTTTATGAATGAACTGCATAAAATCCCATTGCCACCGTGGGCCTTAATTGCCATAGCC
ATAGTTGCGGTCCTTCTAGTCGTGACCTGCTGCTTCTGTGTCTGTAAGAAATGTTTGTTCAAAAAGAAAA
ACAAGAAGAAGGGAAAGGAAAAGGGAGGGAAGAACGCCATTAACATGAAAGACGTGAAAGACTTAGGGAA
GACCATGAAGGATCAGGCCCTTAAGGATGACGATGCTGAAACTGGACTGACTGATGGAGAAGAAAAGGAG
GAGCCCAAGGAAGAGGAGAAACTGGGAAAGCTTCAATATTCACTGGACTATGACTTCCAGAATAACCAGC
TGCTGGTGGGAATCATCCAGGCTGCTGAACTGCCCGCCCTGGACATGGGAGGCACATCTGATCCATACGT
CAAAGTCTTCCTGCTGCCCGACAAAAAGAAGAAGTTTGAGACAAAAGTCCACCGGAAAACCCTCAATCCA
GTCTTCAATGAACAGTTTACTTTCAAGGTGCCATACTCGGAATTAGGTGGCAAGACACTGGTGATGGCTG
TGTATGATTTTGACCGCTTCTCCAAGCACGACATCATTGGAGAGTTCAAAGTTCCTATGAACACCGTGGA
TTTTGGCCACGTCACCGAGGAGTGGCGCGATCTCCAGAGTGCTGAGAAAGAAGAGCAAGAGAAACTGGGT
GACATCTGCTTCTCCCTCCGCTACGTCCCTACTGCCGGCAAGCTGACTGTTGTCATTCTGGAAGCCAAGA
ACCTGAAGAAGATGGATGTGGGTGGCTTATCTGATCCCTATGTAAAGATTCACCTGATGCAGAACGGCAA
GAGACTGAAGAAGAAAAAGACAACGATTAAGAAGAACACACTTAACCCCTACTACAATGAGTCCTTCAGC
TTTGAAGTTCCGTTCGAGCAAATCCAGAAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTG
GCAAGAACGACGCCATCGGCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCAGAGCTGCGACACTGGTC
AGACATGCTGGCCAACCCCCGGCGACCCATCGCCCAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTGAT
GCCATGCTGGCTGTCAAGAAGTAAAGGGGAAAAGAAGCCTTTCTGCGTCTGCCCACATAGTGCTCTTTAG
CCAGTATCTGTAAATACCTCAGTAATATGGGTCCTTTCAGTTTCCAGCCATGCATTCCTGATACAATCCA
GTGGTACTTCAGATCCTGTTTTAATTTGCACAAATTTAAGTGTAGAAAGCCCCTATGCCCTTCATCATAC
CACTGCCCTCCAAATCTACTCTTCTTTTAAGCAATATGATGTGTAGATAGAGCATGACTGAAATGTATTG
TATCACACCGTTGTATATACCAGTATGCTAAAGATTTATTTCTAGTTTGTGTATTTGTATGTTGTAAGCG
TTTCCTAATCTGTGTATATCTAGATGTTTTTAATAAGATGTTCTATTTTAAACTATGTAAATTGACTGAG
ATATAGGAGAACTGATAATATATTATATGGTAAATATAGTATCGTCTGCATTCCAGCAAAAATATCAATT
TGAAAGGCACTAGTACAGTTAAACCAACATCTTAAAGGACAACTTAAACCTGAACTTTCTATTGAATCCT
TTGAGTACCAAGATTTGCTCACATGACATCTTTGATGGGTGAACCCAATTTTGTAGAATTCTTTCACAGG
CAAATAGCATGTTCTGAGCAGCATCCATGCTGACCTCAAGGAAGCAAAGCCACAAACCAGAATAGCATCT
GTCTGTCTGTCTCTGCAAAGCTAAAAAATCATGGCTTCACTCTTCCAGTCAAGGAAGCAACGAACACGAA
GGAGCCAGTGAGTTCCTACGACTGCATCTAGCATAGCATAACCGTGGTGTTCTCTGTGTGTGCGTGTGCA
AGTGTGAAAGTGTATGCACGAGTCTTATGTGTGGTGCATGCCTTTGTTCGGGGTTGGGGTGGGAGAGGAG
AAGCTGAGAGAAGTCAGCGTTTCTGAAATGTTGCCTGACTACTTAAACAGAAACTAATAGCTCTCCAGTA
TCACATTTATATAACACGTGCAACCTGGGAATTCTGGTCCAGATTTCACCCCAATATTGATTCCAAAAGG
TATGCACGTGAGACTTTGTAACAAAATATTTTATTATATAAAACCAGATTAGAAGGAATGCAGAATATTT
TTAACACAGCAATCTGTGCTTATTCCACAACATTACTTTGTGGTAAACAGACAGTATTGTAATCCCATGA
AAAGATGAAATATAACAATTAGCCATAGTTCTGAATGCACTTCGATTAAGCCAAAACAGACAGCTAGTGA
TCTTTTTATATGCTCTTTTTACTTAAGTTTTAATTTGTCCTTTAAACAAAGGTGAAAAAAAAAACCAAGA
ACAAGTTCTAGCAAACTGAAGCAACCTCTTATGTACACTAGATGCTTGACTTAGGAGGAGTTTTTAAATG
TTCTCAATGTTATTCTGTAGTAAATGGCACTATTATGAAGCCACTAGTCATTCCATATGAGTCTTAAGGA
CTGCTCTGTGTAACACTGTGACTGCCCTGTGTGCTTAGACACGTAGTTTCCTCAGTGGCTAGCACCCAAC
TTACTCCGTAGTGATATTGTAACAATACTGCCATTCCCTCGTACTGCACTGCCCAATGTGTGTGTAGCAC
AAACAGTTCTCATTCTTAAGGACCAATTCAGAACTGAACAGCTATGCATAGGACAGAAAGATACATAGAC
TGGGGGGGTGGGGAGAACACACAGCATCTTGTCAACACTGTGCACTAGTCCACATTTGTCTCGCTGAGGT
AGACAGCCACTTCAGGAAGGTGAGCCTGCTACCTAACACTGCTTCTAGACTCTTCGCCCACTTGATAGTG
CGGCCTGTTTTCACCTCCAGGTCACAGAAAACAGCAACATCCTGACGGGAGAGGACCATCTTCACATCTA
CCAAAATAAAATGGAGGCATGGAAAGTGTGGCCTCCTGTTTGATCTATAGGATTTAGCTCCATGCTTTGG
ACAAAAGTCAAGAGAATCCCAGAAAGGGCTCTTGGTAGACTTTAAACATGGGGTTGGCCGTTCCTCCTGC
ACACACAACACAATCTCCAAACCCTGGTTCTTCCCTCATTGGAGAAGAGGCAAAGCCTTTGCTCCTCACC
TCCATCCCAGCAAACTCTTCAGAATTAGAACAGATGCAGAGGACAAGGAGAAAATCCTTTCCTGTGGACG
AACAGCACATTTTCAACATTAAAACCACAGATGATAGGAAAGGCACACTCATAGGTGAGATTAAACAGGA
GACTCAAATGAGGGGCTTTCCTACTTAAGGGACCAAGTCCTACCAAAGAGAAGGAGTTGACTTTAAAAAA
AAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5365461 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365461 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001257 same experiment
 EMAGE:21512 same embryo
 EMAGE:21507 same embryo
 EMAGE:21509 same embryo
 EMAGE:21510 same embryo
 EMAGE:21508 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS