EMAGE mouse gene expression spatial database

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EMAGE:21512

Ddhd1 DDHD domain containing 1 ( MGI:2150302)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001239. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001239_01	euxassay_001239_02	euxassay_001239_03	euxassay_001239_04

euxassay_001239_05	euxassay_001239_06	euxassay_001239_07	euxassay_001239_08	euxassay_001239_09

euxassay_001239_10	euxassay_001239_11	euxassay_001239_12	euxassay_001239_13	euxassay_001239_14

euxassay_001239_15	euxassay_001239_16	euxassay_001239_17	euxassay_001239_18	euxassay_001239_19

euxassay_001239_20	euxassay_001239_21	euxassay_001239_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21512_wholemount_strong.wlz

21512_wholemount_moderate.wlz

21512_wholemount_weak.wlz

21512_wholemount_possible.wlz

21512_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21512_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot mesenchyme

weak

regional

weak expression: see section 2 3 4 5 0 1

thymus primordium

weak

homogeneous

weak expression: see section 1 2 3 4 5

submandibular gland primordium

strong

regional

strong expression: see section 7 8 9 0 6 7 8 9

vibrissa

weak

regional

weak expression: see section 5 6 7 0 1

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 6 7 8 9 0 1 2 4 5 6 7 8 9 0 weak expression: see section 5 1

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 0 1 2 5 6 7 8

facial vii ganglion

moderate

regional

moderate expression: see section 4 5 1

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 7 8 9

trigeminal v ganglion

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 8 9 0 1 2

vagus x ganglion

moderate

regional

moderate expression: see section 9 8

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 6 7 8 8 9

dorsal root ganglion

moderate

regional

moderate expression: see section 8 9 0 5 6 7 8

external naris epithelium

moderate

regional

moderate expression: see section 2 5 6

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 8 9 0 1 2 5 6 7 8 9

esophagus

weak

regional

weak expression: see section 2 3 4

lower jaw incisor

moderate

regional

moderate expression: see section 1 2 5 6

lower jaw molar

moderate

regional

moderate expression: see section 6 7 9 0

upper jaw incisor

moderate

regional

moderate expression: see section 1 2 6

upper jaw molar

moderate

regional

moderate expression: see section 6 7 9 0

liver lobe

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5399
Entity Detected:	Ddhd1, DDHD domain containing 1 ( MGI:2150302)
Sequence:	sense strand is shown >T5399 ATTGTCTGTCACTGGTAAATTTAGATATTATTTGTCTTTGTTCCTTTCAATAAAAGTAGAGCATATATAT TTGACAGACATTAACTAATTTTTGCTTTACTGGTCTTTTAACTAGGAGTTGCATTTTAGAAAGCATTTAG AATAATGTTTGTCAAGTGCAGTTTCTAAAAGTTAGGGCTAAAAGGCAGAGGCAGAATAGAAAACCTCACT TAAATTTCCTTTGAAGCTCAGTGTTTTTGCTTTCTTAGTTGTCTACCACCTCCCTCCCTTCTCCCTCCCT TCTCTGTTCAAATGGAGAGGATATAAGGAGAGCACAGATGCCACAGGTCTTAAGCGAAAGCGTTCCCAAG CTGTTCACAGTTTCAAGCTGAGTCGGAACCACGTGGACTGGCACAGCATGGATGAAGTCTATCTCTACAG TGACGCAACAACATCCAAAATTGCGAGAACCGTTACCCAAAAACTGGGCTTTTCTAAAGCCTCCAGCAGT GGGACCAGACTGCACAGAGGTTATGTAGAAGAAGCCACACTGGAAGACAAGCCGTCGCAGACTTCTCATA TCGTGTTTGTTGTGCACGGCATTGGACAGAAGATGGACCAAGGAAGAATCATCAAAAACACAGCCATGAT GAGAGAGGCTGCAAGGAAAATGGAAGAAAAGCATTTTTCCAACCATGCAACACATGTTGAATTTCTGCCT GTTGAATGGCGATCAAAGCTTACTCTTGATGGAGACACAGTTGATTCCATCACTCCTGACAAAGTGCGAG GCCTGAGGGACATGCTCAACAGCAGCGCGATGGACATCATGTACTACACCAGCCCACTCTACAGGGATGA GCTAGTTAAAGGCCTTCAGCAAGAGCTGAATCGATTATATTCCCTTTTCTGTTCCCGGAATCCAGACTTT GAAGAGAAAGGGGGTAAAGTCTCAATAGTGTCCCATTCCTTGGGGTGTGTAATCACTTATGACATCATGA TGGGCTGGAATCCAGGTGGACTCTATGAGCAGCTGCTGCAGAAGGAGGAAGAGCTGCCCGACGAGCGGTG GATGAGCTATGAAGAGCGGCATCTTCTTGACGAGCTCTATATAACAAAGCGACGGCTGAGGGAAATAGAA GATCGGCTGCACGGATTGAAGGCGCCATCCATATCACAAACACCTGCCTTAAAATTTAAGGTTGAAAATT TCTTCTGTATGGGATCTCCGCTAGCAGTTTTTTTGGCATTACGCGGCATCCGACCAGGAAATTCTGGAAG TCAAGACCACATTTTACCTAGAGAGATCTGTAACCGCTTACTAAATATTTTTCATCCTACGGACCCAGTG GCCTACAGATTAGAACCGTTGATTTTGAAACACTACAGCAACATTTCTCCAGTCCAGATCCACTGGTACA ACACTTCAAACCCTCTACCTTATGAGCATATGAAGCCAAATTTTCTCAACCCAGCAAAAGAACCTACCTC AGTTTCAGACAGTGAGAACATAGCAGCTATCCCAAGCCCTGTGACCTCCCCCGTCTTATCTCGGCGCCAC TATGGAGAATCTATAACTAACATTGGCAAAGCAAGCATATTGGGGGCTGCTAGCATTGGAAAGGGCCTTG GAGGAATGTTGTTCTCAAGATTTGGACGTTCTTCAGCATCACAGCCGTCTGAGCCATCTAAAGACTCACT GGAAGATGATAAGAAGCCTTCAGCTTCACCCTCTACCACCACCGTGGCAACGCAGACCCTGCCGCACAGT GGCTCCGGCTTCCTCGACTCCGCATATTTCAGACTTCAAGAATCGTTCTTTTATCTCCCACAACTTCTTT TTCCGGAAAATGTAATGCAGAGTAAAGATGATAGCCTCGTGGAACTGGAACACAGGATCGACTTTGAACT CAGAGAAGGCCTTGTGGAGAGCCGCTATTGGTCAGCCGTCACGTCGCACACTGCCTATTGGTCATCCTTG GATGTCGCTCTTTTTCTTTTAACATTCATGTACAAACACGAGCATGATACCGAGGCCAAGCCCAGCCTAG GTTCACTCTGAACTCTTGAAGGACGTGAATGCCCAAAGCTTTTCTGTTAAACATGTGTCAAGATGCAGAG ACTTCAAGGTTCCAGTTTTGTTAAGGGCAAGAACTATTTTAATTTAAAAGCACTTTATTTTATTTTTAAA AGAGAAAAAAACCACATGTTCAGTTCTAAAGGAGTTATTTATGTTTCTGTCGTTTTGATTCTGTCTGGCA GAGCCCTGCAAAGAATCACCAGAGTAGAAGTGTGGTGGGCTTAGGAGAACTGCATGAAGGCAGCCATCAC AGTCCAGGTCTCCAGAGGAGCCTAATGTAGCGTTTTAACCAAAAGGCGTCACAGCACTAACAGCCCTAAG TCCAGTACACTCTGCATAGACTACAAGGTGGCATCATGGTTTCTTGATTCTGTGAAGGCTTCCGTGTCCG CAGTTCCTTTGAAAGAAGAGTATAACAAATTTTAAATGTTCTAAATTTAACTTGTTTTGAAAAACTAATG CTAAAAAGCAATATTTGAACTACTGCACTTATAATTTATAATCTCTACTTATTCAGCATATGAAACATTT TTAATATTTCTTTTAAGCCACACTTATTTTGTATCATGTTGAGGCCCCTTTTTCTCAAAACTCCATCTTT GTAAATGAGCAGATGATCCTGTGACGTACTTTCAGGGTTTTTTTGTTTGTTTGTTTTGGTTTGGTTTTTT CTTTTTGTTTTTTTTGTTGTTGTTTTATTTTTGTTTTCTTTTCTTTTTCATTAAAAACATGGTTTCACAC AGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365658 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365658 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001239 same experiment
	EMAGE:21507 same embryo
	EMAGE:21509 same embryo
	EMAGE:21511 same embryo
	EMAGE:21510 same embryo
	EMAGE:21508 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source