EMAGE mouse gene expression spatial database

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EMAGE:21525

Nfs1 nitrogen fixation gene 1 (S. cerevisiae) ( MGI:1316706)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001010_01	euxassay_001010_02	euxassay_001010_03	euxassay_001010_04	euxassay_001010_05

euxassay_001010_06	euxassay_001010_07	euxassay_001010_08	euxassay_001010_09	euxassay_001010_10

euxassay_001010_11	euxassay_001010_12	euxassay_001010_13	euxassay_001010_14	euxassay_001010_15

euxassay_001010_16	euxassay_001010_17	euxassay_001010_18	euxassay_001010_19	euxassay_001010_20

euxassay_001010_21	euxassay_001010_22	euxassay_001010_23	euxassay_001010_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21525_wholemount_strong.wlz

21525_wholemount_moderate.wlz

21525_wholemount_weak.wlz

21525_wholemount_possible.wlz

21525_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21525_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 3 4 5 6 7

liver lobe

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3941
Entity Detected:	Nfs1, nitrogen fixation gene 1 (S. cerevisiae) ( MGI:1316706)
Sequence:	sense strand is shown >T3941 GCTGAGGACTTGCAAGCGCCGCGGATGGTGGGCTCTGTGGCTGGGAACATGCTGCTGCGAGCCGCTTGGA GGCGGGCGTCGTTGGCGGCTACCTCCTTGGCCCTGGGAAGGTCCTCGGTGCCCACCCGGGGACTGCGCCT GCGCGTTGTAGATCATGGCCCCCATTCGCCTGTTCACTCAGAGGCAGAGGCAGTGCTGCGACCTCTCTAC ATGGACGTACAGGCCACCACTCCTCTGGATCCCAGAGTGCTTGATGCCATGCTCCCATACCTTGTCAACT ACTATGGGAACCCTCATTCTCGGACTCATGCATATGGCTGGGAGAGCGAGGCAGCCATGGAACGTGCTCG CCAGCAAGTAGCATCTCTGATTGGAGCTGATCCTCGGGAGATCATTTTCACTAGTGGAGCTACTGAGTCC AACAACATAGCAATTAAGGGAGTGGCCAGGTTCTACAGGTCAAGGAAAAAACACTTGGTCACCACCCAGA CAGAACACAAATGTGTGCTGGATTCCTGCCGCTCCCTGGAAGCTGAGGGCTTTCGGGTCACTTACCTCCC TGTGCAGAAGAGCGGGATCATTGACTTAAAGGAACTAGAGGCTGCCATCCAGCCAGACACCAGCCTGGTC TCCGTTATGACTGTGAACAATGAAATTGGAGTAAAGCAACCGATTGCAGAAATAGGGCAGATTTGTAGTT CCAGAAAGGTGTACTTCCACACTGATGCCGCCCAAGCGGTTGGGAAAATCCCACTTGACGTCAATGACAT GAAGATTGATCTCATGAGCATCAGCGGTCACAAACTCTATGGCCCTAAAGGGGTTGGTGCTATCTATATC CGCCGTAGGCCCCGGGTACGTGTGGAGGCCCTACAGAGTGGAGGCGGGCAGGAGCGGGGTATGCGGTCTG GGACAGTGCCCACACCCTTGGTGGTGGGCCTGGGAGCTGCATGTGAGTTGGCACAACAAGAGATGGAATA CGACCATAAGCGGATCTCAAAGTTAGCAGAGCGACTGGTACAGAAGATAATGAAGAACCTTCCAGATGTG GTAATGAACGGGGACCCGAAGCAGCACTACCCTGGCTGTATCAACCTCTCCTTCGCGTATGTGGAAGGAG AGAGCCTGCTGATGGCCCTCAAGGATGTGGCCTTGTCCTCAGGGAGTGCCTGCACTTCTGCGTCATTGGA GCCCTCTTACGTCCTCAGAGCAATTGGTACCGATGAGGATTTAGCACACTCCTCTATCAGGTTTGGCATC GGCCGCTTCACTACAGAAGAGGAGGTGGACTACACGGCGGAGAAGTGCATCCACCACGTGAAGCGCCTTC GAGAAATGAGTCCTCTCTGGGAGATGGTCCAGGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAGCA TTAGAGGAGTGGGCTGTGACTTTGTGCTGGCCTGGCTGTTCTGGCCTCACTGATCTGTGCACAGCCAGGA ACCTCACTGCCTGACGTGGTCCATGCTCAAAGCAGATGGCAAGCTCTGCAGTGACTTCACCACAGAATGT TCTCTAGTGAAAGCTGAGGCTAGCTTTCCTAGCCTCGGCTCCTGTGTAGGCATGTATTCCGTAATCTTCC TCAGGAGTTCTTCTCTTGCCTGGCGTGGCCATTCTTATCTGAGGGCCTGGAGGCACTGCCTTGTTACTGC CACAGGAGGACCTACCTGTGCAGAGATGGAAGAACTGGAAGAAGGCTCTAGGTTCAAAGACTGCTTCTCT GTGAATCATTACTCTAGCTGCTCAGCTGAACTGGCGCCATTAGGGACTGGCATAAACAAACTTTATTTTA ACCTTTTTTGTGGCTTTGAAAGACATCTCTTTGTATCGACTTCAGCTGAAGAACACCAAGTTGTATTAAA GTCTTGTTTCCTTTGGGTTCTCATCTTCTTTCCTCTCTTTTGACTTAATGTAAGTAGAAATATCCATGAC CCCAATCATTATTCTAATAATTTACTTAATTCATTAAACTTAATTCAATAAATTCCCAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:3711352 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3711352 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001010 same experiment
	EMAGE:21527 same embryo
	EMAGE:21526 same embryo
	EMAGE:21524 same embryo
	EMAGE:21528 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source