EMAGE mouse gene expression spatial database

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EMAGE:21527

Evx1 even skipped homeotic gene 1 homolog ( MGI:95461)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001002_01	euxassay_001002_02	euxassay_001002_03	euxassay_001002_04	euxassay_001002_05

euxassay_001002_06	euxassay_001002_07	euxassay_001002_08	euxassay_001002_09	euxassay_001002_10

euxassay_001002_11	euxassay_001002_12	euxassay_001002_13	euxassay_001002_14	euxassay_001002_15

euxassay_001002_16	euxassay_001002_17	euxassay_001002_18	euxassay_001002_19	euxassay_001002_20

euxassay_001002_21	euxassay_001002_22	euxassay_001002_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21527_wholemount_strong.wlz

21527_wholemount_moderate.wlz

21527_wholemount_weak.wlz

21527_wholemount_possible.wlz

21527_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21527_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 8 9 0 6 7 8

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 1 2 4 5

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 4 5 6 7 9 9 0

pons mantle layer

moderate

regional

moderate expression: see section 0 1 2 4 5 6 7 8

midbrain mantle layer

moderate

regional

moderate expression: see section 7 8 9 7 8

ventral grey horn

weak

regional

weak expression: see section 2 3 4 5 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5305
Entity Detected:	Evx1, even skipped homeotic gene 1 homolog ( MGI:95461)
Sequence:	sense strand is shown >T5305 CAGCTCCAGCCTTCTCCCTTCCGGTTTGACATTGGCAGGGAAGGGGCAGGGGCTCCAGGGTCCCTAGGCA GCTTCTGTGGGGCGAACCTCTTCTCCCTTAACCCAGCACACAGCCTGATTAGCAAGTGATGGGTGAGGAG GGGTTTTTGAATGTTGAATGTATGTATAATAATGATCACCACTCTGCTGGGCCACCAAGCCCGGAGCTGC TGAGCCGGTCTAACAAGGCGCCCTGGGAAGAGCTTAGGGAACGGAGACTTCTTACATTCTTCTCTCATTG TCTCCCCAAATTGCCACAGGGCCTTGGCTTTCAGCTGCCAGTACAAACCTTCAGCGCCTCTGGAGGACCC TGTCTCTCCCCTTCACTGGGGTTTATT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GACTTCTTTCCCTATTTCTGG; Reverse Primer - name:unspecified, sequence:CATTGGATGATTGAAGTTGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001002 same experiment
	EMAGE:21525 same embryo
	EMAGE:21526 same embryo
	EMAGE:21524 same embryo
	EMAGE:21528 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source