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EMAGE:21586

Syt8 synaptotagmin VIII ( MGI:1859867)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586
"Pseudo-wholemount" of euxassay_001971. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001971_01 euxassay_001971_02 euxassay_001971_03 euxassay_001971_04
EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586
euxassay_001971_05 euxassay_001971_06 euxassay_001971_07 euxassay_001971_08 euxassay_001971_09
EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586
euxassay_001971_10 euxassay_001971_11 euxassay_001971_12 euxassay_001971_13 euxassay_001971_14
EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586
euxassay_001971_15 euxassay_001971_16 euxassay_001971_17 euxassay_001971_18 euxassay_001971_19
EMAGE:21586 EMAGE:21586 EMAGE:21586 EMAGE:21586
euxassay_001971_20 euxassay_001971_21 euxassay_001971_22 euxassay_001971_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21586Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21586_wholemount_strong.wlz
21586_wholemount_moderate.wlz
21586_wholemount_weak.wlz
21586_wholemount_possible.wlz
21586_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21586_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
bladder
strong strong
regionalstrong expression: see section 1 2 3
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3986
Entity Detected:Syt8, synaptotagmin VIII ( MGI:1859867)
Sequence:sense strand is shown

>T3986
TGGCCTCGAGCCAGATTCGGACGAGGGTCCCACCCACCTGGCAACTAATCTGTTCCCGCTGTCCCCCGGG
ACCGGCGACGTCCCAGGGGACCCACGAGGGAGCGGGCGCAACGCGTGGCTGTGGGTGGGAGCCATGGACG
CCTTCATCCGAGTCGCCAACCAAAGCCAGGGCCGGGACCGACTTTTCAGGGTTCAGACTGGGCAACGTGT
TCCATGCCATCCAGGCAACTGAGCAGAGCATCCAAGCCGCTGACCTCGCGCCCCGCTTATGCCTGACATT
AGCCAACCTCAACCGCGTGGTTTATTACATCTGTGACACTGTCCTCTGGGCGAAGAGTGTGGGTCTTACC
TCTGGAGTCAACAGAGAGAAATGGCAAAGGTGGGCGGCTCGCCATTACTACTACTTCCTCTTGCTGAGCC
TGGTCCGGGATCTGTATGAGATCTTGCTGCAGATGGGACAAGTTGCACGTGACAGAGCAAAGAGAGAGAA
ATCCTCTCGGGACCCTCCTAAGTACAGCGTGGCTAATGAAGAGACTGAATGGCTCCAGTCCTTCCTC
Notes:The probe template was PCR amplified from IMAGE:457048 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:457048 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001971 same experiment
 EMAGE:21590 same embryo
 EMAGE:21588 same embryo
 EMAGE:21587 same embryo
 EMAGE:21589 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS