EMAGE mouse gene expression spatial database

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EMAGE:21590

Efemp2 epidermal growth factor-containing fibulin-like extracellular matrix protein 2 ( MGI:1891209)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001935. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001935_01	euxassay_001935_02	euxassay_001935_03	euxassay_001935_04

euxassay_001935_05	euxassay_001935_06	euxassay_001935_07	euxassay_001935_08	euxassay_001935_09

euxassay_001935_10	euxassay_001935_11	euxassay_001935_12	euxassay_001935_13	euxassay_001935_14

euxassay_001935_15	euxassay_001935_16	euxassay_001935_17	euxassay_001935_18	euxassay_001935_19

euxassay_001935_20	euxassay_001935_21	euxassay_001935_22	euxassay_001935_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21590_wholemount_strong.wlz

21590_wholemount_moderate.wlz

21590_wholemount_weak.wlz

21590_wholemount_possible.wlz

21590_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21590_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T586
Entity Detected:	Efemp2, epidermal growth factor-containing fibulin-like extracellular matrix protein 2 ( MGI:1891209)
Sequence:	sense strand is shown >T586 TCCTCGAGNCTGTTGGCCTACTGGAGTGAGCTGCGCGGTCCGTCCGTCCGTCCGCCCGCCCGCAGGCACT GCTGGAGTCGACGCCCTCAGGATGCTCCCTTTTGCCTCCTGCCTCCCCGGGTCTTTGCTGCTCTGGGCGT TTCTGCTGTTGCTCTTGGGAGCAGCGTCCCCACAGGATCCCGAGGAGCCGGACAGCTACACGGAATGCAC AGATGGCTATGAGTGGGATGCAGACAGCCAGCACTGCCGGGATGTCAACGAGTGCCTGACCATCCCGGAG GCTTGCAAGGGTGAGATGAAATGCATCAACCACTACGGGGGTTATTTGTGTCTGCCTCGCTCTGCTGCCG TCATCAGTGATCTCCATGGTGAAGGACCTCCACCGCCAGCGGCCCATGCTCAACAACCAAACCCTTGCCC GCAGGGCTACGAGCCTGATGAACAGGAGAGCTGTGTGGATGTGGACGAGTGTACCCAGGCTTTGCATGAC TGTCGCCCTAGTCAGGACTGCCATAACCTTCCTGGCTCCTACCAGTGCACCTGC
Notes:	The probe template was PCR amplified from IMAGE:1885437 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885437 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001935 same experiment
	EMAGE:21588 same embryo
	EMAGE:21587 same embryo
	EMAGE:21586 same embryo
	EMAGE:21589 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source