EMAGE mouse gene expression spatial database

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EMAGE:21591

Alas1 aminolevulinic acid synthase 1 ( MGI:87989)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009192_01	euxassay_009192_02	euxassay_009192_03	euxassay_009192_04	euxassay_009192_05

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euxassay_009192_11	euxassay_009192_12	euxassay_009192_13	euxassay_009192_14	euxassay_009192_15

euxassay_009192_16	euxassay_009192_17	euxassay_009192_18	euxassay_009192_19	euxassay_009192_20

euxassay_009192_21	euxassay_009192_22	euxassay_009192_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21591_wholemount_strong.wlz

21591_wholemount_moderate.wlz

21591_wholemount_weak.wlz

21591_wholemount_possible.wlz

21591_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21591_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

regional

strong expression: see section 3 4 5 0 1 2 3

liver

strong

single cell

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3519
Entity Detected:	Alas1, aminolevulinic acid synthase 1 ( MGI:87989)
Sequence:	sense strand is shown >T3519 TGGCCTCGAGCCAGATTCGGACGAGGGACTCCACCAATGCTGCTGGCTGGAGCCCTGGAGTCTGTGCGGA TCCTGAAGAGCAGCGAGGGGCGCGCCCTTCGCCGCCAGCACCAGCGAAATGTCAAGCTTCTGAGGCAGAT GCTAATGGACGCTGGCCTCCCGGTCATCCACTGTCCGAGTCACATCATCCCTGTGCGGGTTGCCGATGCT GCTAAGAACACAGAGATCTGTGATGAGTTGATGACCAGGCATAATGTCTACGTCCAGGCCATCAATTACC CAACAGTGCCTCGCGGGGAGGAGCTCCTGCGGATCGCCCCCACCCCGCATCACACCCCGCAGATGATGAA CTTCTTCGTGGAGAAGCTGCTGGTCACGTGGAAGCGAGTTGGGCTGGAACTTAAGCCACATTCGTCAGCT GAATGCAACTTCTGCAGGAGGCCCCTGCACTTTGAAGTGATGAGCGAAAGAGAGAAAGCCTATTTCTCAG GCATGAGCAAGATGGTGTCTGCCCAGGCCTGACTCTGACTCAGTTATTCACAAACCCCAGGCCATTACCA TACCC
Notes:	The probe template was PCR amplified from IMAGE:337593 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:337593 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009192 same experiment
	EMAGE:21594 same embryo
	EMAGE:21595 same embryo
	EMAGE:21593 same embryo
	EMAGE:21592 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source