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EMAGE:21593

Ezr ezrin ( MGI:98931)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21593 EMAGE:21593 EMAGE:21593 EMAGE:21593 EMAGE:21593
euxassay_009206_01 euxassay_009206_02 euxassay_009206_03 euxassay_009206_04 euxassay_009206_05
EMAGE:21593 EMAGE:21593 EMAGE:21593 EMAGE:21593 EMAGE:21593
euxassay_009206_06 euxassay_009206_07 euxassay_009206_08 euxassay_009206_09 euxassay_009206_10
EMAGE:21593 EMAGE:21593 EMAGE:21593 EMAGE:21593 EMAGE:21593
euxassay_009206_11 euxassay_009206_12 euxassay_009206_13 euxassay_009206_14 euxassay_009206_15
EMAGE:21593 EMAGE:21593 EMAGE:21593 EMAGE:21593 EMAGE:21593
euxassay_009206_16 euxassay_009206_17 euxassay_009206_18 euxassay_009206_19 euxassay_009206_20
EMAGE:21593 EMAGE:21593 EMAGE:21593
euxassay_009206_21 euxassay_009206_22 euxassay_009206_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21593Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21593_wholemount_strong.wlz
21593_wholemount_moderate.wlz
21593_wholemount_weak.wlz
21593_wholemount_possible.wlz
21593_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21593_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 2 3 4 5 6 7 8 9 3 4 5 6 7 8 9 0
thymus primordium
strong strong
homogeneousstrong expression: see section 8 9 0 1 2
sublingual gland primordium
strong strong
regionalstrong expression: see section 9 5
submandibular gland primordium
strong strong
regionalstrong expression: see section 6 7 8 6 7 8
thyroid gland
strong strong
regionalstrong expression: see section 2 3
pituitary gland
moderate moderate
regionalmoderate expression: see section 9 0 1 2 3
epidermis
strong strong
regionalstrong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3
vibrissa
strong strong
regionalstrong expression: see section 6 7 8 9 0 1 2 3
hypothalamus ventricular layer
strong strong
homogeneousstrong expression: see section 9 0 1 2 3
diencephalon lateral wall ventricular layer
strong strong
homogeneousstrong expression: see section 9 0 1 2
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 5 6 7 8 9 0 1 2 3 4 5 6 7
olfactory cortex ventricular layer
strong strong
homogeneousstrong expression: see section 0 1 2 6 7
telencephalon ventricular layer
strong strong
homogeneousstrong expression: see section 1 2 3 4 5 6 7 8 9 0 1 3 4 5 6 7 8 9 0 1
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 6 7 8 9 1 2 moderate expression: see section 0
rest of cerebellum marginal layer
strong strong
regionalstrong expression: see section 2 5 6 1 2 3 4 5 moderate expression: see section 3 4 7 0 6 7 8
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 2 3 4 6 7 weak expression: see section 3 4
pons ventricular layer
strong strong
regionalstrong expression: see section 5 6 7 8 9 1 2 3 4 5 moderate expression: see section 4 0 6
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8
midbrain ventricular layer
strong strong
regionalstrong expression: see section 5 6 8 9 1 2 moderate expression: see section 4 7 0
spinal cord ventricular layer
strong strong
regionalstrong expression: see section 7 8 9
dorsal root ganglion
weak weak
regionalweak expression: see section 5 6 9 0 1 2 3 4
saccule
moderate moderate
regionalmoderate expression: see section 5 6 6 7
cochlea
moderate moderate
regionalmoderate expression: see section 3 4 5 6 7 5 6 7 8 9
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 1 2 3 moderate expression: see section 1 2 3 4 8 9 0
cornea epithelium
strong strong
regionalstrong expression: see section 5 2 3 moderate expression: see section 1 2 3 4
lens
strong strong
regionalstrong expression: see section 1 3
heart ventricle
weak weak
homogeneousweak expression: see section 6 7 8 9 0 1 2 3 4 5
esophagus
strong strong
regionalstrong expression: see section 9 0 1
stomach
strong strong
regionalstrong expression: see section 1 2 3 4 5 6 7
hindgut
strong strong
regionalstrong expression: see section 9 0 1
rectum
strong strong
regionalstrong expression: see section 9
midgut
strong strong
regionalstrong expression: see section 8 9 0 1 2 3 4 5 6 7
lower jaw incisor
strong strong
regionalstrong expression: see section 2 3 6 7
lower jaw molar
strong strong
regionalstrong expression: see section 7 8 8
oral epithelium
strong strong
regionalstrong expression: see section 6 7 8 9 0 1 2 4 5 7 8 9 0
palatal shelf epithelium
strong strong
regionalstrong expression: see section 9 0 1 2 3 4 6
upper jaw incisor
strong strong
regionalstrong expression: see section 1 2 3 5 6 7
upper jaw molar
strong strong
regionalstrong expression: see section 7 8 9
liver lobe
weak weak
regionalweak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1
urinary system
strong strong
spottedstrong expression: see section 1 5 6
bladder
strong strong
regionalstrong expression: see section 9 0
kidney calyx
strong strong
regionalstrong expression: see section 3 4 5 6 2 3 4
kidney pelvis
strong strong
regionalstrong expression: see section 5 6 2
glans of male genital tubercle
strong strong
regionalstrong expression: see section 2 3
urethra of male
strong strong
regionalstrong expression: see section 9 0
larynx
strong strong
regionalstrong expression: see section 0 1 2
lung
strong strong
regionalstrong expression: see section 2 3 4 5 6 7 9 0 1 2 3 4 5 6 7 8 moderate expression: see section 1 8
trachea
strong strong
regionalstrong expression: see section 0 1
axial skeleton
moderate moderate
regionalmoderate expression: see section 7 8
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 9 0 1 2 3 4
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3524
Entity Detected:Ezr, ezrin ( MGI:98931)
Sequence:sense strand is shown

>T3524
TGGCCTCGAGCCAGATTCGGACGAGGCCNAGCTCTCCAGTGAGGGCATCCTGGATGACCGCAACGAGGAG
AAGCGGATCACAGAGGCAGAGAAGAATGAGCGCGTGCAGCGGCAGCTGCTGACCCTGAGCAATGAGTTGT
CCCAGGCCCGGGATGAGAACAAGAGGACCCACAATGACATCATCCACAACGAGAACATGCGGCAAGGCAG
GGACAAGTATAAGACGCTGCGGCAAATCAGGCAGGGCAACACCAAGCAACGCATTGACGAGTTCGAGGCC
ATGTAGAGGCCAGGCTGGGACCAAGGGCAGAGGGCACCTCACTGCAGGCAGGTGTCACACTTGGCTCTTT
AGTTCTCTTAAGTTTAGACACCCCCTTGCTGTGTTCCAGTCCCTTAAAGAGCAGTTACGGGGCCTGCATT
CTGCCCCGAGACCCAGTGGGCTCCTCCTTGGTTCCTTCTAATTGTATCACATAGTGCCAAACAGGTCAGA
TTTAATGACAGTTACCGAATCACTTCCTGTTTGGAGCAGGGATTCGGAGGGCTGGC
Notes:The probe template was PCR amplified from IMAGE:3166365 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3166365 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009206 same experiment
 EMAGE:21594 same embryo
 EMAGE:21591 same embryo
 EMAGE:21595 same embryo
 EMAGE:21592 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS