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EMAGE:21599

Epor erythropoietin receptor ( MGI:95408)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21599 EMAGE:21599 EMAGE:21599 EMAGE:21599 EMAGE:21599
euxassay_003299_01 euxassay_003299_02 euxassay_003299_03 euxassay_003299_04 euxassay_003299_05
EMAGE:21599 EMAGE:21599 EMAGE:21599 EMAGE:21599 EMAGE:21599
euxassay_003299_06 euxassay_003299_07 euxassay_003299_08 euxassay_003299_09 euxassay_003299_10
EMAGE:21599 EMAGE:21599 EMAGE:21599 EMAGE:21599 EMAGE:21599
euxassay_003299_11 euxassay_003299_12 euxassay_003299_13 euxassay_003299_14 euxassay_003299_15
EMAGE:21599 EMAGE:21599 EMAGE:21599 EMAGE:21599
euxassay_003299_16 euxassay_003299_17 euxassay_003299_18 euxassay_003299_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21599Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21599_wholemount_strong.wlz
21599_wholemount_moderate.wlz
21599_wholemount_weak.wlz
21599_wholemount_possible.wlz
21599_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21599_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver and biliary system
strong strong
regionalstrong expression: see section 1
liver
strong strong
regionalstrong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3452
Entity Detected:Epor, erythropoietin receptor ( MGI:95408)
Sequence:sense strand is shown

>T3452
GGCCCGGGCAGCTCCTTCCCTGAGGATCCACCTGCCCACCTAGAGGTCCTCTCAGAGCCACGCTGGGCAG
TGACTCAGGCTGGGGACCCAGGGGCAGATGATGAGGGGCCCTTACTGGAGCCGGTGGGCAGTGAGCATGC
CCAGGACACCTACTTGGTATTGGATAAGTGGTTGCTGCCCCGGACCCCATGCAGTGAGAACCTCTCAGGG
CCTGGGGGCAGTGTGGACCCTGTGACTATGGATGAAGCTTCAGAAACATCTTCCTGCCCGTCTGACTTGG
CCTCAAAGCCCAGGCCAGAGGGCACCTCACCTTCCAGCTTTGAGTACACCATCCTGGACCCCAGCTCTCA
GCTCCTGTGCCCTCGGGCACTGCCTCCCGAGCTACCTCCCACTCCACCTCACTTGAAGTACCTATACCTT
GTGGTGTCCGATTCTGGCATCTCAACAGATTACAGTTCGGGGGGCTCTCAGGGAGTCCACGGGGACTCAT
CTGATGGCCCCTACTCCCACCCCTATGAGAACAGCCTTGTCCCAGACTCAGAGCCTCTG
Notes:The probe template was PCR amplified from IMAGE:3026171 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3026171 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003299 same experiment
 EMAGE:21596 same embryo
 EMAGE:21598 same embryo
 EMAGE:21597 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS