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EMAGE:21619

Actb actin, beta ( MGI:87904)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619
"Pseudo-wholemount" of euxassay_007714. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007714_01 euxassay_007714_02 euxassay_007714_03 euxassay_007714_04
EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619
euxassay_007714_05 euxassay_007714_06 euxassay_007714_07 euxassay_007714_08 euxassay_007714_09
EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619
euxassay_007714_10 euxassay_007714_11 euxassay_007714_12 euxassay_007714_13 euxassay_007714_14
EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619
euxassay_007714_15 euxassay_007714_16 euxassay_007714_17 euxassay_007714_18 euxassay_007714_19
EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619 EMAGE:21619
euxassay_007714_20 euxassay_007714_21 euxassay_007714_22 euxassay_007714_23 euxassay_007714_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21619Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21619_wholemount_strong.wlz
21619_wholemount_moderate.wlz
21619_wholemount_weak.wlz
21619_wholemount_possible.wlz
21619_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21619_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35873
Entity Detected:Actb, actin, beta ( MGI:87904)
Sequence:sense strand is shown

>T35873
CACACCTTCTACAATGAGCTGCGTGTGGCCCCTGAGGAGCACCCTGTGCTGCTCACCGAGGCCCCCCTGA
ACCCTAAGGCCAACCGTGAAAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCAGCCATGTACGT
AGCCATCCAGGCTGTGCTGTCCCTGTATGCCTCTGGTCGTACCACAGGCATTGTGATGGACTCCGGAGAC
GGGGTCACCCACACTGTGCCCATCTACGAGGGCTATGCTCTCCCTCACGCCATCCTGCGTCTGGACCTGG
CTGGCCGGGACCTGACAGACTACCTCATGAAGATCCTGACCGAGCGTGGCTACAGCTTCACCACCACAGC
TGAGAGGGAAATCGTGCGTGACATCAAAGAGAAGCTGTGCTATGTTGCTCTAGACTTCGAGCAGGAGATG
GCCACTGCCGCATCCTCTTCCTCCCTGGAGAAGAGCTATGAGCTGCCTGACGGCCAGGTCATCACTATTG
GCAACGAGCGGTTCCGATGCCCTGAGGCTCTTTTCCAGCCTTCCTTCTTGGGTATGGAATCCTGTGGCAT
CCATGAAACTACATTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89476. Forward Primer - name:089476_F_cDNA_Actb, sequence:CACACCTTCTACAATGAGCTGC; Reverse Primer - name:089476_N_SP6_cDNA_Actb, sequence:GAATGTAGTTTCATGGATGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007714 same experiment
 EMAGE:21621 same embryo
 EMAGE:21618 same embryo
 EMAGE:21620 same embryo
 EMAGE:30445 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS