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EMAGE:21621

Acvr1b activin A receptor, type 1B ( MGI:1338944)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621
"Pseudo-wholemount" of euxassay_007715. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007715_01 euxassay_007715_02 euxassay_007715_03 euxassay_007715_04
EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621
euxassay_007715_05 euxassay_007715_06 euxassay_007715_07 euxassay_007715_08 euxassay_007715_09
EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621
euxassay_007715_10 euxassay_007715_11 euxassay_007715_12 euxassay_007715_13 euxassay_007715_14
EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621
euxassay_007715_15 euxassay_007715_16 euxassay_007715_17 euxassay_007715_18 euxassay_007715_19
EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621 EMAGE:21621
euxassay_007715_20 euxassay_007715_21 euxassay_007715_22 euxassay_007715_23 euxassay_007715_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21621Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21621_wholemount_strong.wlz
21621_wholemount_moderate.wlz
21621_wholemount_weak.wlz
21621_wholemount_possible.wlz
21621_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21621_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
sublingual gland primordium
weak weak
regionalweak expression: see section 0
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 6 7 8 9 7 8 9
brain
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4
vibrissa
moderate moderate
regionalmoderate expression: see section 2 3 4 5 6 6 7 8 9 0 1 3
facial vii ganglion
strong strong
regionalstrong expression: see section 5 6 7 1 2
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 8 9 0
trigeminal v ganglion
strong strong
regionalstrong expression: see section 4 5 6 7 8 9 7 8 9 0 1 2 3
vagus x ganglion
strong strong
regionalstrong expression: see section 9 moderate expression: see section 8 9
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 7 9 1 moderate expression: see section 8 8 9 0
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 9 6
spinal cord
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 6 7 8
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 9 0 1 2 3 6 7 8 9 0
neural retina
moderate moderate
regionalmoderate expression: see section 1 2 3 2 3 4
anterior naris
moderate moderate
regionalmoderate expression: see section 9 0 2 3
external naris
moderate moderate
regionalmoderate expression: see section 9 3 4
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 7 8 9 0 1 3 4 5 6 7
esophagus
moderate moderate
regionalmoderate expression: see section 3
stomach
moderate moderate
regionalmoderate expression: see section 2 3 4 5 6 7 8 9
rectum
moderate moderate
regionalmoderate expression: see section 4
midgut
moderate moderate
regionalmoderate expression: see section 8 9 0 1 2 3 4 5 6 7 8
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 9 0 5
lower jaw molar
moderate moderate
regionalmoderate expression: see section 7 8
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 9 0
upper jaw molar
moderate moderate
regionalmoderate expression: see section 6 8
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35876
Entity Detected:Acvr1b, activin A receptor, type 1B ( MGI:1338944)
Sequence:sense strand is shown

>T35876
ATCGTCTTCCTGGTCATCAACTATCACCAGCGTGTCTACCATAACCGCCAGAGGTTGGACATGGAGGACC
CCTCTTGCGAGATGTGTCTCTCCAAAGACAAGACGCTCCAGGATCTCGTCTACGACCTCTCCACGTCAGG
GTCTGGCTCAGGGTTACCCCTTTTTGTCCAGCGCACAGTGGCCCGAACCATTGTTTTACAAGAGATTATC
GGCAAGGGCCGGTTCGGGGAAGTATGGCGTGGTCGCTGGAGGGGTGGTGACGTGGCTGTGAAAATCTTCT
CTTCTCGTGAAGAACGGTCTTGGTTCCGTGAAGCAGAGATCTACCAGACCGTCATGCTGCGCCATGAAAA
CATCCTTGGCTTTATTGCTGCTGACAATAAAGATAATGGCACCTGGACCCAGCTGTGGCTTGTCTCTGAC
TATCACGAGCATGGCTCACTGTTTGATTATCTGAACCGCTACACAGTGACCATTGAGGGAATGATTAAGC
TAGCCTTGTCTGCAGCCAGTGGTTTGGCACACCTGCATATGGAGATTGTGGGCACTCAAGGGAAGCCGGG
AATTGCTCATCGAGACTTGAAGTCAAAGAACATCCTGGTGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 73532. Forward Primer - name:073532_F_cDNA_Acvr1b, sequence:ATCGTCTTCCTGGTCATCAACT; Reverse Primer - name:073532_N_SP6_cDNA_Acvr1b, sequence:TTCACCAGGATGTTCTTTGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007715 same experiment
 EMAGE:21619 same embryo
 EMAGE:21618 same embryo
 EMAGE:21620 same embryo
 EMAGE:30445 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS