EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21639

Capza2 capping protein (actin filament) muscle Z-line, alpha 2 ( MGI:106222)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003331. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003331_01	euxassay_003331_02	euxassay_003331_03	euxassay_003331_04

euxassay_003331_05	euxassay_003331_06	euxassay_003331_07	euxassay_003331_08	euxassay_003331_09

euxassay_003331_10	euxassay_003331_11	euxassay_003331_12	euxassay_003331_13	euxassay_003331_14

euxassay_003331_15	euxassay_003331_16	euxassay_003331_17	euxassay_003331_18	euxassay_003331_19

euxassay_003331_20	euxassay_003331_21	euxassay_003331_22	euxassay_003331_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21639_wholemount_strong.wlz

21639_wholemount_moderate.wlz

21639_wholemount_weak.wlz

21639_wholemount_possible.wlz

21639_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21639_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 3 4 5 6 7

submandibular gland primordium

weak

regional

weak expression: see section 8 9 0 1 9 0

vibrissa

weak

regional

weak expression: see section 5 6 7 0 1

medulla oblongata basal plate

moderate

regional

moderate expression: see section 9 0 1 7 8

metencephalon basal plate

moderate

regional

moderate expression: see section 8 8

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 8 9 0 9 0

trigeminal v ganglion

moderate

regional

moderate expression: see section 4 5 6 7 8 9 0 8 9 0 1 2

ventral grey horn

moderate

regional

moderate expression: see section 2 3 5 8 9 weak expression: see section 4

dorsal root ganglion

moderate

regional

moderate expression: see section 9 0 1 3 4 8 9 weak expression: see section 2 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6378
Entity Detected:	Capza2, capping protein (actin filament) muscle Z-line, alpha 2 ( MGI:106222)
Sequence:	sense strand is shown >T6378 CTCCCAAATTTTCTAATTTACAACTTGTTAAGAATGACAATATACAAAGAAAGTATTTTAAAATAGTGTA TGATTCACTGGTGATTTTTTTTTTACTCAAATAAATACCAAATGTTGCCATTTTAATGTCACACTGATAT TCAGTTTCCAATCCAAGGGGAGGGAGAATCAACTGAGACATGTTCATGAGTTCAGGATTATATATATATT ACAATCTGCCTTATAATACACTTGTGGCTTTGTGATAAAAATAACTCAGGAACATATGGAATTCAAGCTA ATTTGCATAACTGTCACAAGAAAAAAGCATTAAATGCATTTCTGAAATAAGTATTCTCATTTAATTTCAG AATCTCAAAACAGCAACAGACCTTGGCCTTGTTTTAAAAAAGTTTAGTTAGACACTAAGCTAGCTAAATA ACCTTGCTGAGAAGGTTTAACGTAACTCGTTAGAAAATGCTCTCCCTCTAATCTCCATCTTCCATGCTGG AAGAGGTGAAAGGAAAAGGTAGACAATTTCTTTAGCAGCATACATGGCTAAGTCCATCAACCACCTTAAC TAGAGTGCCCATTACTGACTGACCCCACTAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GAATGCATAAATGAAAATTGC; Reverse Primer - name:unspecified, sequence:TTATGATGCATAGGAAAATGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003331 same experiment
	EMAGE:21641 same embryo
	EMAGE:21640 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source