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EMAGE:21640

Steap2 six transmembrane epithelial antigen of prostate 2 ( MGI:1921301)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21640 EMAGE:21640 EMAGE:21640 EMAGE:21640 EMAGE:21640
"Pseudo-wholemount" of euxassay_003352. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003352_01 euxassay_003352_02 euxassay_003352_03 euxassay_003352_04
EMAGE:21640 EMAGE:21640 EMAGE:21640 EMAGE:21640 EMAGE:21640
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View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21640Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21640_wholemount_strong.wlz
21640_wholemount_moderate.wlz
21640_wholemount_weak.wlz
21640_wholemount_possible.wlz
21640_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21640_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
kidney calyx
strong strong
regionalstrong expression: see section 9 0 1 moderate expression: see section 2 9 0 1 2 3
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6362
Entity Detected:Steap2, six transmembrane epithelial antigen of prostate 2 ( MGI:1921301)
Sequence:sense strand is shown

>T6362
GGGTAGATGATATTACTCCTCCCATGCATAAGCCGAAAGCTAAAACGAATTAAAAAGGGCTGGGAAAAGA
GCCAGTTTCTAGACGAAGGCATGGGAGGAGCGGTTCCTCATCTGTCCCCAGAGAGGGTCACAGTGATGTG
ATGACAAGAGGTGCTCGCAGAAGCCACACACACTTCCATCCATGCCATTACTTTAGGCCCTCGCCTTGGT
CCCTGTCAGCGGGCTGTTGATTGGCAGTCCTGCAGTCTGAAACCTGCAGTAAGTTTTCAGCAGAAGGAGA
GATTGGATTCTCTTCAAGTTAATTTTTATAAATGTCATATTTTTCCAACATGCAATTTTGTAGTCCACAT
CTATTGTTACAATCTAGAAATTTCCTTTGTGCAACTGTAACACTGTTGTTCTTTTAACCATATTGCATAG
TTGGGCACAAATATTTGTACTTAATAATACAGATGAGATATAATGTTTAGAACACTTGGCAAAACAGCAG
AATTAGAAGCTTTTAACATTACTCAGTGGACATGCTCTTTCCCCAAGGCTAAAGATTTTAATTACTATTC
AATTTAAGGAGCAGAAATGCATAATCATATATTATTGAGAAGGGGCACATCACATTAGCATCTAGGGTAT
GCAGGAGCAATCTTATGCTCTCATAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTTTACACACCACCAAACTTC; Reverse Primer - name:unspecified, sequence:ATGTCGATAAAATCCACACAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003352 same experiment
 EMAGE:21639 same embryo
 EMAGE:21641 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS