EMAGE mouse gene expression spatial database

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EMAGE:21641

Sept8 septin 8 ( MGI:894310)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003350. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003350_01	euxassay_003350_02	euxassay_003350_03	euxassay_003350_04

euxassay_003350_05	euxassay_003350_06	euxassay_003350_07	euxassay_003350_08	euxassay_003350_09

euxassay_003350_10	euxassay_003350_11	euxassay_003350_12	euxassay_003350_13	euxassay_003350_14

euxassay_003350_15	euxassay_003350_16	euxassay_003350_17	euxassay_003350_18	euxassay_003350_19

euxassay_003350_20	euxassay_003350_21	euxassay_003350_22	euxassay_003350_23	euxassay_003350_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21641_wholemount_strong.wlz

21641_wholemount_moderate.wlz

21641_wholemount_weak.wlz

21641_wholemount_possible.wlz

21641_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21641_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

dermis

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

ventral grey horn

weak

regional

weak expression: see section 2 3 7 8 9

spinal cord ventricular layer

weak

regional

weak expression: see section 7

nose

moderate

regional

moderate expression: see section 0 7 weak expression: see section 7 8 9 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6337
Entity Detected:	Sept8, septin 8 ( MGI:894310)
Sequence:	sense strand is shown >T6337 GAGTTACACCTTGTGACCCTTCTCCCATAACATGGTGTGAGGACGGACTGGGAGCCGGTACAGACTCCAG TGTTTACAGCTTTGCCATGTCCCCACCTGCTGGCCTCAGGCTGCCTGGGCCTGGCCAGCTATCCTTCTCT ATGCAAATGCGTCAAAGCCATGACTGCTGGAACCCAAAACTGACAAGGTTTATTTTTTCCAGAGCCGGTG GCTGGTCTTCCATTTACAGTGTCACTATTCCTTGATGGAGCAGTTATGTGCCCGCTCTGGTGATGGCCCC AGCCAGTGATGCTAGGCCTAATTGTTCATCATCAAGCCGGCGACTCATGTGGTGCCTGCCCTAGGCGTGG CCTGCGGTCTGGCAGATCCGTGCTACAGAATTCGCCTGGTTCCTCTCTATTTTAATTTTTCTAACCTAGA GCTTAATTTCAATAACTTTTTCAACAGTTCTAAATTTTTATTTTTGGCACCAGTATAAAGACAAATAATA TCTATCGCTCCCATTATTTTCATAAGTAACACAGATTCCCTGATTTTT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACAGACATACATGTCGAGAGC; Reverse Primer - name:unspecified, sequence:GACAGTCTCAGTCTCAAGTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003350 same experiment
	EMAGE:21639 same embryo
	EMAGE:21640 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source