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EMAGE:21651

Hbb-y hemoglobin Y, beta-like embryonic chain ( MGI:96027)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21651 EMAGE:21651 EMAGE:21651 EMAGE:21651 EMAGE:21651
"Pseudo-wholemount" of euxassay_004286. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004286_01 euxassay_004286_02 euxassay_004286_03 euxassay_004286_04
EMAGE:21651 EMAGE:21651 EMAGE:21651 EMAGE:21651 EMAGE:21651
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EMAGE:21651 EMAGE:21651 EMAGE:21651 EMAGE:21651 EMAGE:21651
euxassay_004286_10 euxassay_004286_11 euxassay_004286_12 euxassay_004286_13 euxassay_004286_14
EMAGE:21651 EMAGE:21651 EMAGE:21651 EMAGE:21651 EMAGE:21651
euxassay_004286_15 euxassay_004286_16 euxassay_004286_17 euxassay_004286_18 euxassay_004286_19
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euxassay_004286_20 euxassay_004286_21 euxassay_004286_22 euxassay_004286_23 euxassay_004286_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21651Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21651_wholemount_strong.wlz
21651_wholemount_moderate.wlz
21651_wholemount_weak.wlz
21651_wholemount_possible.wlz
21651_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21651_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6366
Entity Detected:Hbb-y, hemoglobin Y, beta-like embryonic chain ( MGI:96027)
Sequence:sense strand is shown

>T6366
ACTTTACTGCTGAGGAAAAGACCCTCATCAATGGCCTGTGGAGTAAGGTCAATGTTGAAGAGGTTGGTGG
TGAAGCCTTGGGAAGGCTTCTTGTTGTGTACCCATGGACCCATAGATTTTTTGACAGCTTTGGGAACTTG
TCCTCTGCTTCTGCCATAATGGGCAACCCAAGGGTCAAAGCCCATGGCAAGAAGGTGCTGACTGCTTTTG
GAGAGTCCATTAAGAATCTAGACAACCTCAAGTCTGCCTTGGCCAAGCTCAGTGAACTGCACTGTGACAA
GCTACATGTGGATCCTGAGAACTTCAAACTCTTGGGTAATGTGCTGGTGATTGTTTTGGCTAGTCACTTC
GGCAATGAATTCACAGCTGAGATGCAGGCTGCCTGGCAGAAGCTGGTGGCTGGGGTGGCCACTGCGCTGT
CCCACAAGTACCACTGAGCCCTCTCTCTAGCTGTCCAGCAATCCTGTGTGTCCGCTATGCCTCCTTTCTG
CACATGAATACTGGACTGTTCTATAGTGTC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CATACTTGCTTCTGACACTCC; Reverse Primer - name:unspecified, sequence:AACAGTCCAGTATTCATGTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004286 same experiment
 EMAGE:21649 same embryo
 EMAGE:21652 same embryo
 EMAGE:21648 same embryo
 EMAGE:21650 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS