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EMAGE:21652

Ostm1 osteopetrosis associated transmembrane protein 1 ( MGI:2655574)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21652 EMAGE:21652 EMAGE:21652 EMAGE:21652 EMAGE:21652
"Pseudo-wholemount" of euxassay_004289. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004289_01 euxassay_004289_02 euxassay_004289_03 euxassay_004289_04
EMAGE:21652 EMAGE:21652 EMAGE:21652 EMAGE:21652 EMAGE:21652
euxassay_004289_05 euxassay_004289_06 euxassay_004289_07 euxassay_004289_08 euxassay_004289_09
EMAGE:21652 EMAGE:21652 EMAGE:21652 EMAGE:21652 EMAGE:21652
euxassay_004289_10 euxassay_004289_11 euxassay_004289_12 euxassay_004289_13 euxassay_004289_14
EMAGE:21652 EMAGE:21652 EMAGE:21652 EMAGE:21652 EMAGE:21652
euxassay_004289_15 euxassay_004289_16 euxassay_004289_17 euxassay_004289_18 euxassay_004289_19
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euxassay_004289_20 euxassay_004289_21 euxassay_004289_22 euxassay_004289_23 euxassay_004289_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21652Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21652_wholemount_strong.wlz
21652_wholemount_moderate.wlz
21652_wholemount_weak.wlz
21652_wholemount_possible.wlz
21652_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21652_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6346
Entity Detected:Ostm1, osteopetrosis associated transmembrane protein 1 ( MGI:2655574)
Sequence:sense strand is shown

>T6346
CCTCCTTAACAGTTTTCAGAGAGTGATTCCCATTATGCATTTATCCTTGTTAAAGGTTTACAGTAACTGA
GGTTCTAATATGACTTTTATAAATACTATTTTACATCTTATTTTTGTCTTTATTTAGTAAGTAATTTATA
ATCACTGGACTGCTTAATTACCTTTGAGGACAAGATGGATTCATCTTATGCCAGGGATTTGCATCATGAA
TTTCATTAAGTTATTTGGCAACCTGTAACTTGTTAGTAGTTCAAGTCGAATGTCACCCAAGTGTGTCATA
CTGTGTTTAAATTTGTGATTTTTTTTAATGAAAATTTTATCTTGGAATATTTGGAGATTTGGGGAGAAAC
AAGGACAAACACAAGAGCTTAAATTTCAGAAAATAGACAGGGACCTGAGGGATGCTCACGGTGAGACAGC
TGCGTGGTTTACACTGGAGATGACTCGGTTGACAGGCTCGCACAGGAAGCCTCCCAGTTACGGGAAAGAT
GAAAGTCACATGACTGAAACGAAATTACCCATCTCACTGTCAGGAAACTAGTTCTTCTTTGGCATATTTC
TAGCAACCTTTAAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTCTCTAGTTCCCTGAAGTCC; Reverse Primer - name:unspecified, sequence:ATGTCACTTCACTCACTTTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004289 same experiment
 EMAGE:21649 same embryo
 EMAGE:21651 same embryo
 EMAGE:21648 same embryo
 EMAGE:21650 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS