EMAGE mouse gene expression spatial database

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EMAGE:21660

Myh6 myosin, heavy polypeptide 6, cardiac muscle, alpha ( MGI:97255)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018788. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018788_01	euxassay_018788_02	euxassay_018788_03	euxassay_018788_04

euxassay_018788_05	euxassay_018788_06	euxassay_018788_07	euxassay_018788_08	euxassay_018788_09

euxassay_018788_10	euxassay_018788_11	euxassay_018788_12	euxassay_018788_13	euxassay_018788_14

euxassay_018788_15	euxassay_018788_16	euxassay_018788_17	euxassay_018788_18	euxassay_018788_19

euxassay_018788_20	euxassay_018788_21	euxassay_018788_22	euxassay_018788_23	euxassay_018788_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21660_wholemount_strong.wlz

21660_wholemount_moderate.wlz

21660_wholemount_weak.wlz

21660_wholemount_possible.wlz

21660_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21660_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 moderate expression: see section 7 8 4 6

vertebral axis musculature

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

extrinsic ocular muscle

strong

regional

strong expression: see section 1 2 3 6 7 8 9 moderate expression: see section 4 5 6 0 1

heart atrium

strong

homogeneous

strong expression: see section 4 5 6 7 8 9 0 1 2 3 4 5 6 7

heart ventricle

strong

homogeneous

strong expression: see section 5 6 7 8 9 0 1 2 3 4

tongue muscle

strong

regional

strong expression: see section 8 9 0 1 2 3 moderate expression: see section 7

tail mesenchyme

strong

regional

strong expression: see section 7 8 9 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50700
Entity Detected:	Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha ( MGI:97255)
Sequence:	sense strand is shown >T50700 GGTCCACATTCTTCAGGATTCTCTGAAAAGTTAACCAGAGTTTGAGTGACAGAATGACGGACGCCCAGAT GGCTGACTTCGGGGCAGCAGCCCAGTACCTCCGAAAGTCAGAGAAGGAACGCCTAGAGGCCCAGACACGG CCCTTTGACATCCGCACGGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGTTAAGGCCAAGGTCGTGT CCCGGGAAGGGGGCAAAGTCACTGCGGAAACTGAAAACGGAAAGACGGTGACCATAAAGGAGGACCAGGT GATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGACCTTCCTGCACGAGCCG GCTGTGCTGTACAACCTCAAGGAGCGCTACGCGGCCTGGATGATCTATACCTACTCGGGCCTCTTCTGCG TCACCGTCAACCCCTACAAGTGGCTGCCAGTGTACAATGCGGAAGTG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_018788 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source