EMAGE mouse gene expression spatial database

EMAGE:21690

Taf1d TATA box binding protein (Tbp)-associated factor, RNA polymerase I, D ( MGI:1922566)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017944. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017944_01	euxassay_017944_02	euxassay_017944_03	euxassay_017944_04

euxassay_017944_05	euxassay_017944_06	euxassay_017944_07	euxassay_017944_08	euxassay_017944_09

euxassay_017944_10	euxassay_017944_11	euxassay_017944_12	euxassay_017944_13	euxassay_017944_14

euxassay_017944_15	euxassay_017944_16	euxassay_017944_17	euxassay_017944_18	euxassay_017944_19

euxassay_017944_20	euxassay_017944_21	euxassay_017944_22	euxassay_017944_23

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50967
Entity Detected:	Taf1d, TATA box binding protein (Tbp)-associated factor, RNA polymerase I, D ( MGI:1922566)
Sequence:	sense strand is shown >T50967 TGAATGTGATATCAAGTTGGTTGAAGACAGTTGCTTCATAATAAGTTCAGAATTCTCAAGGAAGAGAAAC TTGGAACAAGAGAAGATTAAGAAAGAATCTACATTTTCTAAAAAAGCCAAAGATGCCACACACAGAGAGA AAGGGCACAGAAGAACCTTGAAAGGAAATGAACATGTGACTATTGAAGAAGGCTTGTCTTGATTATGAAG TCAGAATTGGAAGATACCCATGAATGGATGTTTGTTTCAGTGTTGCACTGGCACTTCCTTCATAAAGATT AGAGTTGTGAGTGTGAGGAATGGATATGAGAAGACCCTAAAGAGATGTTTGGGTGACACGAGTGAACCCT ACCCTAAAATGGTTTAAAGATAACATGAAGGGTCCTTAAAATAAATTGAAGAAATCCTCAAATCTTCTGG TAAGATGTTAACTCTGAAACCACATGTGAGATGAGGTGACCTGGATGGACAGCAAAGGAATCAAATCCCT CAAATAATTTATTTGACTTAAATTAAAAATAAATAAATATATGCAGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_017944 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source