EMAGE mouse gene expression spatial database

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EMAGE:21702

Wnt2 wingless-related MMTV integration site 2 ( MGI:98954)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007181. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007181_01	euxassay_007181_02	euxassay_007181_03	euxassay_007181_04

euxassay_007181_05	euxassay_007181_06	euxassay_007181_07	euxassay_007181_08	euxassay_007181_09

euxassay_007181_10	euxassay_007181_11	euxassay_007181_12	euxassay_007181_13	euxassay_007181_14

euxassay_007181_15	euxassay_007181_16	euxassay_007181_17	euxassay_007181_18	euxassay_007181_19

euxassay_007181_20	euxassay_007181_21	euxassay_007181_22	euxassay_007181_23	euxassay_007181_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21702_wholemount_strong.wlz

21702_wholemount_moderate.wlz

21702_wholemount_weak.wlz

21702_wholemount_possible.wlz

21702_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21702_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

mandible

weak

regional

weak expression: see section 8 9 0 1 3 4 5 6 7 8 9 0

maxilla

moderate

regional

moderate expression: see section 2 3 4 weak expression: see section 0 1

bladder

strong

regional

strong expression: see section 2 3 4 5

lung

strong

regional

strong expression: see section 1 2 3 4 5 6 7 9 0 1 2 3 4 5 7 8 9 0 1 2 3 4 moderate expression: see section 8 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50022
Entity Detected:	Wnt2, wingless-related MMTV integration site 2 ( MGI:98954)
Sequence:	sense strand is shown >T50022 CTCCCTCTGCTCTTGACCTGGCTCACCCCTGAGGTCAGCTCTTCATGGTGGTACATGAGAGCTACAGGTG GCTCCTCCAGGGTGATGTGTGACAATGTGCCAGGCCTGGTGAGCCGGCAGCGTCAGCTGTGCCACCGACA CCCAGATGTGATGCGTGCCATTGGCCTGGGTGTGGCTGAGTGGACTGCAGAGTGCCAACACCAGTTCCGC CAGCATCGCTGGAACTGCAACACCCTGGACAGAGATCACAGCCTCTTTGGCCGGGTCCTCCTCCGAAGTA GTCGGGAATCGGCCTTTGTTTACGCCATCTCTTCAGCTGGCGTTGTATTTGCCATCACCAGGGCCTGTAG CCAAGGAGAATTAAAGTCCTGCTCCTGTGATCCAAAGAAGAAAGGAAGTGCCAAGGACA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CTCCCTCTGCTCTTGACCTG; Reverse Primer - name:unspecified, sequence:TGTCCTTGGCACTTCCTTTC. The reverse primer contains a 5' extension containing an T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using T7 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007181 same experiment
	EMAGE:21701 same embryo
	EMAGE:21700 same embryo
	EMAGE:21699 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source