Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21703

Ascl2 achaete-scute complex homolog 2 (Drosophila) ( MGI:96920)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21703 EMAGE:21703 EMAGE:21703 EMAGE:21703 EMAGE:21703
euxassay_007175_01 euxassay_007175_02 euxassay_007175_03 euxassay_007175_04 euxassay_007175_05
EMAGE:21703 EMAGE:21703 EMAGE:21703 EMAGE:21703 EMAGE:21703
euxassay_007175_06 euxassay_007175_07 euxassay_007175_08 euxassay_007175_09 euxassay_007175_10
EMAGE:21703 EMAGE:21703 EMAGE:21703 EMAGE:21703 EMAGE:21703
euxassay_007175_11 euxassay_007175_12 euxassay_007175_13 euxassay_007175_14 euxassay_007175_15
EMAGE:21703 EMAGE:21703 EMAGE:21703 EMAGE:21703 EMAGE:21703
euxassay_007175_16 euxassay_007175_17 euxassay_007175_18 euxassay_007175_19 euxassay_007175_20
EMAGE:21703 EMAGE:21703 EMAGE:21703
euxassay_007175_21 euxassay_007175_22 euxassay_007175_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21703Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21703_wholemount_strong.wlz
21703_wholemount_moderate.wlz
21703_wholemount_weak.wlz
21703_wholemount_possible.wlz
21703_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21703_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 6 7 4 5 6 7 8 9 0 1 2 3
foot
moderate moderate
regionalmoderate expression: see section 3
fibula
moderate moderate
regionalmoderate expression: see section 3
tibia
moderate moderate
regionalmoderate expression: see section 3
mandible
moderate moderate
regionalmoderate expression: see section 5 6 7 4 5 7 8 9 0 1 2
palatal shelf
moderate moderate
regionalmoderate expression: see section 0 1
axial skeleton
moderate moderate
regionalmoderate expression: see section 5 6 7 8 9 0 1 2 3 4 5 6
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 8 9 0 1 2 3 4 5
pectoral girdle and thoracic body wall skeleton
moderate moderate
regionalmoderate expression: see section 1 2 3 0 1 2
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 4 5 6 5 8 9 2
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50003
Entity Detected:Ascl2, achaete-scute complex homolog 2 (Drosophila) ( MGI:96920)
Sequence:sense strand is shown

>T50003
GAAGGTGCAAACGTCCACTTCCCACCGCACCCGGTTCCTCGCGAGCACTTTTCCTGTGCCGCACCAGAAC
TCGTAGCAGGGGCCCAGGGGCTGAATGCAAGCTTGATGGACGGCGGCGCGCTGCCCAGACTCATGCCCAC
CTCGTCTGGAGTCGCTGGAGCCTGCGCTGCTCGGCGGAGACAAGCGTCTCCGGAATTGCTGCGCTGCAGC
CGGCGGCGGCGATCTGGAGCAACCGAGGCCAGCAGCAGCTCGGCTGCCGTGGCACGCCGCAATGAGCGCG
AGCGCAACCGCGTAAAGCTGGTAAACTTGGGCTTCCAGGCGCTGCGGCAGCACGTGCCGCACGGCGGCGC
CAACAAGAAGCTGAGTAAGGTGGAGACGCTGCGCTCCGCGGTAGAGTACATTCGTGCGCTGCAGCGGCTG
CTCGCAGAGCACGACGCGGTGCGTGCCGCGCTCGCTGGGGGGCTGTTAACACCCGCTACTCCGCCGTCCG
ATGAGTGCGCGCAGCCCTCTGCCTCCCCTGCCAGCGCGTCTCTGTCCTGCGCCTCTACGTCTCCGTCCCC
GGACCGCCTGGGCTGCTCTGAGCCTACCTCCCCGCGCTCCGCCTACTCGTCGGAGGAAAGCAGCTGCGAG
GGAGAGCTAAGCCCGATGGAGCAGGAGCTGCTTGACTTTTCC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GAAGGTGCAAACGTCCACTTC; Reverse Primer - name:unspecified, sequence:GGAAAAGTCAAGCAGCTCCTG. The reverse primer contains a 5' extension containing an T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using T7 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007175 same experiment
 EMAGE:21704 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS