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EMAGE:21704

Uncx UNC homeobox ( MGI:108013)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704
euxassay_019456_01 euxassay_019456_02 euxassay_019456_03 euxassay_019456_04 euxassay_019456_05
EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704
euxassay_019456_06 euxassay_019456_07 euxassay_019456_08 euxassay_019456_09 euxassay_019456_10
EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704
euxassay_019456_11 euxassay_019456_12 euxassay_019456_13 euxassay_019456_14 euxassay_019456_15
EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704
euxassay_019456_16 euxassay_019456_17 euxassay_019456_18 euxassay_019456_19 euxassay_019456_20
EMAGE:21704 EMAGE:21704 EMAGE:21704 EMAGE:21704
euxassay_019456_21 euxassay_019456_22 euxassay_019456_23 euxassay_019456_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21704Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21704_wholemount_strong.wlz
21704_wholemount_moderate.wlz
21704_wholemount_weak.wlz
21704_wholemount_possible.wlz
21704_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21704_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 0 1 5 6 7 weak expression: see section 9 2
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 1 3 4 5 6 weak expression: see section 8 9 2 7
diencephalon lateral wall marginal layer
moderate moderate
regionalmoderate expression: see section 0
telencephalon mantle layer
weak weak
regionalweak expression: see section 2 3 4 5 6 9 0 1 2 3
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 5 2 weak expression: see section 3 4 6 7 8 1
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 0 1 4 5 weak expression: see section 7 8 9 2 6 7
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 0 1 3 4 5 weak expression: see section 6 7 8 9 2 6 7 9 0
dorsal grey horn
weak weak
regionalweak expression: see section 9 0 1 2 3 4
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 4 5 6 7 weak expression: see section 0 1 8
metanephros
strong strong
regionalstrong expression: see section 7 8 moderate expression: see section 5 6 7 8 9 9 weak expression: see section 0 5 6
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55208
Entity Detected:Uncx, UNC homeobox ( MGI:108013)
Sequence:sense strand is shown

>T55208
AAACGCAAACACGAGAAGAAACTGCTCAAGAGTCAGAGCCGCCACCTGCACTCGCCCGGTGGCCTGTCCC
TGCACAGCGCGCCCAGCTCCGACAGCGACAGCGGTGGCGGAGGCCTGTCTCCAGAGCCACCCGAGCCGCC
ACCGCCGACCGCCGCGGCCAAGGGTCCTGGAGCGCACGGCTCTGGCATCGCGGGTTCCGCTCCGGTACCT
CCTGGCGAGCCACCCGCGCCTGGCACCTGCGATCCCGCCTTCTACCCGAGCCAAAGAAGCGGCGCCGGCT
CGCAGCCACGGCTAGGTCGCCCGGCGGACAAGGACACGGTCCCTTGCGGGCCTGGAGCGGCGGCGACTGC
GGGCCTGCCTAAGGCTAGCCCGTTCAGCGTGGAGAGCCTGCTGTCCGACTCGCCACCGCGCCGCAAAGCC
ACCCCAGCCAACGCTGCGGCCACTGCGGGGCTGGACTTCACGCCGGGGCTGCCGTGTGCACCTCGGACCC
TGATTGGCAAGGGCCACTTCTTGCTCTACCCCATCACGCAGCCCCTGGGTTTCCTGGTGCCACAGGCAGC
GCTCAAGGGCGGAGCGGGCCCTGAATTGGTGCCCAAGGACGCGCCGCCCGCGCCCCCCGCGCCCCCCGCA
CCACCCGCGCAGGCCAGTTTCGGGACCTTCCCCGGACCTGGTGGCGCTGCGGACCCGGCCTTCGCCCGTC
GCAGCCCTGAGGTTGTTGCCTCTCCCGGGCCACCCGCTCCCGCTTCTTTCCGGGACCTAACA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AAACGCAAACACGAGAAGAAA; Reverse Primer - name:unspecified, sequence:TGTTAGGTCCCGGAAAGAAGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_019456 same experiment
 EMAGE:21703 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS