EMAGE mouse gene expression spatial database

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EMAGE:21707

Gli3 GLI-Kruppel family member GLI3 ( MGI:95729)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018378. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018378_01	euxassay_018378_02	euxassay_018378_03	euxassay_018378_04

euxassay_018378_05	euxassay_018378_06	euxassay_018378_07	euxassay_018378_08	euxassay_018378_09

euxassay_018378_10	euxassay_018378_11	euxassay_018378_12	euxassay_018378_13	euxassay_018378_14

euxassay_018378_15	euxassay_018378_16	euxassay_018378_17	euxassay_018378_18	euxassay_018378_19

euxassay_018378_20	euxassay_018378_21	euxassay_018378_22	euxassay_018378_23	euxassay_018378_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21707_wholemount_strong.wlz

21707_wholemount_moderate.wlz

21707_wholemount_weak.wlz

21707_wholemount_possible.wlz

21707_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21707_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 1 phalanx

moderate

regional

moderate expression: see section 3 4 5 6

forelimb digit 2 phalanx

moderate

regional

moderate expression: see section 3 4 5 6

forelimb digit 3 phalanx

moderate

regional

moderate expression: see section 3 4 5

hand mesenchyme

moderate

regional

moderate expression: see section 1

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 8

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 8 9

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 8 9 0 1

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 9 0 1

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 9 0 1

foot mesenchyme

moderate

regional

moderate expression: see section 7 weak expression: see section 6

hypothalamus ventricular layer

weak

regional

weak expression: see section 5 6 7

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 6 7 8

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 5 6 1 2 3

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 5 6 7 8 9 0 1 3 4 5 6 7 8 0 1 2 3 4 weak expression: see section 2

midbrain ventricular layer

moderate

regional

moderate expression: see section 8 9 0 1 3 4 weak expression: see section 2 5 6 7 8

axial skeleton tail region

weak

regional

weak expression: see section 6 7 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50449
Entity Detected:	Gli3, GLI-Kruppel family member GLI3 ( MGI:95729)
Sequence:	sense strand is shown >T50449 TTGCTAGATTTCAGCCTGTCCGTGGCACCAAATGAGTTAGCTGGCAACACAGTGAATGGCATGCAAACCC AAGATCAAATGGGACAGGGATACATTGCCCATCAGCTACTCAGTGGCAGCATGCAACACCAGGGGCCCAG CCGCCCTGGTCAACAGGTACTAGGGCAGGTTGGTGCTACCTCACATATCAACATCTATCAAGGGACAGAG AGCTGCCTACCAGGGACTCAGGACAACAGCAGCCAGCCATCAAGCATGGCAGCTATCAGGGGCTACCAGC CCTGTGCCAGCTATGGGGGTAACAGGCGTCAGGCAATGCCAAGGGGCAACCTCACTCTGCAACAAGGACA GCTCAGTGACATGAGTCAGAGCAGCAGGGTGAACAGCATCAAAATGGAGGCACAAGGTCAGTCCCAGCAG CTCTGCTCTACCGTGCAGAATTATTCCGGTCAGTTCTATGACCAAACCATGGGCTTCAGTCAGCAAGACA GGAAAGCTGGCTCGTTCTCCCTCTCAGATGCCAACTGCCTGCTCCAAGGGACATGCACTGAAAACTCTGA GTTACTCTCCCCAGGTGCTAACCAGGTAACAAGCACAGTTGACAGCTTTGAGAGTCATGACCTAGAAGGT GTGCAGATTGATTTTGATGCCATCATAGATGATGGGGACCATACCAGCCTAATGTCAGGGGCCTTGAGCC CAAGTATTATTCAGAACCTTTCCCACAGCTCCTCCCGTCTCACCACTCCGCGGGCATCCCTCCCATTCCC AATCCCTATCCATGGGCACCACCAACATGGCTATCGGGGATATGAGTTCTTTGCTGACCTCCCTTGCAGA AGAAAGCAAGTTCCTTGCAGTTATGCAGTAGGCGGTAGGCAAGGAGGACCACAAACACAAAGACTGAAAT GACTTGGGATTTTTTTTTCTTTTTTAAGTTCTGTGTGTATTTTAGCAATCTCATCTCACCTGATTGGGAT GTGTCTCAAG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTGCTAGATTTCA; Reverse Primer - name:unspecified, sequence:CTTGAGACACATCCC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_018378 same experiment
	EMAGE:21708 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source