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EMAGE:21711

Elf1 E74-like factor 1 ( MGI:107180)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711
"Pseudo-wholemount" of euxassay_019460. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019460_01 euxassay_019460_02 euxassay_019460_03 euxassay_019460_04
EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711
euxassay_019460_05 euxassay_019460_06 euxassay_019460_07 euxassay_019460_08 euxassay_019460_09
EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711
euxassay_019460_10 euxassay_019460_11 euxassay_019460_12 euxassay_019460_13 euxassay_019460_14
EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711
euxassay_019460_15 euxassay_019460_16 euxassay_019460_17 euxassay_019460_18 euxassay_019460_19
EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711 EMAGE:21711
euxassay_019460_20 euxassay_019460_21 euxassay_019460_22 euxassay_019460_23 euxassay_019460_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21711Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21711_wholemount_strong.wlz
21711_wholemount_moderate.wlz
21711_wholemount_weak.wlz
21711_wholemount_possible.wlz
21711_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21711_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
regionalstrong expression: see section 1 4 5 moderate expression: see section 2 3 6
submandibular gland primordium
strong strong
regionalstrong expression: see section 9 moderate expression: see section 7 8 9 0 8 0 1 2
pancreas
weak weak
regionalweak expression: see section 8 9 0
thyroid gland
moderate moderate
regionalmoderate expression: see section 0 weak expression: see section 1 5 6 7
epidermis
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 4 5 7 8 9 0 1 2 3 4
vibrissa
moderate moderate
regionalmoderate expression: see section 5 6 7 8 2 3 weak expression: see section 3 4 1 4
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 1 2 3 moderate expression: see section 4 5 6 7 8 0 2 weak expression: see section 1 3 4
cornea epithelium
moderate moderate
regionalmoderate expression: see section 1 2 3 4 4
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 8 9 0 1 2 4 5 6 7 8 9 0
esophagus
strong strong
regionalstrong expression: see section 3 4 5
stomach
strong strong
regionalstrong expression: see section 9 0 1 2 3 4
hindgut
strong strong
regionalstrong expression: see section 4 5 6
midgut
strong strong
regionalstrong expression: see section 9 0 1 2 4 5 6 7 8 9 0 moderate expression: see section 3
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 1 2 6 7 8 weak expression: see section 5
oral epithelium
strong strong
regionalstrong expression: see section 5 6 7 8 9 0 1 2 4 5 6 7 8 moderate expression: see section 2 weak expression: see section 9 0 1
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 1 2 6 7
liver
strong strong
spottedstrong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4
bladder
strong strong
regionalstrong expression: see section 4 5 moderate expression: see section 6
metanephros
moderate moderate
regionalmoderate expression: see section 9 0 1 9 0 1 weak expression: see section 8 2 8 2
urethra of male
strong strong
regionalstrong expression: see section 5 moderate expression: see section 4 6
larynx
strong strong
regionalstrong expression: see section 3 4 5
lung
strong strong
regionalstrong expression: see section 3 4 5 6 7 8 9 0 1 7 8 9 0 1 2 3 moderate expression: see section 2 3 4 5 6 weak expression: see section 4
trachea
strong strong
regionalstrong expression: see section 3 4
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55036
Entity Detected:Elf1, E74-like factor 1 ( MGI:107180)
Sequence:sense strand is shown

>T55036
TATTACCAAAGAGGTATTCTTGCAAAAGTGGAAGGTCAGCGCTTGGTGTATCAGTTTAAAGAAATGCCAA
AAGATCTTATCTACATAGATGATGAGGATCCAAGTTCCAGCATAGAGTCTTCGGATCAGTCCTTATCGTC
AACAACCGCATCAAGTCGGAATCAAGCAAACCGATCAAGAGTATCTTCAAGTCCAGGAATTAAAGGAGGG
GCCGCTACAATTCTAAAGCCCGGGAATTCTAAAGCTGCAAACCCCAAAGATCCGGTGGAAGTTGGACAAC
CTTCAGAAGTTCTGAGGACAGTGCAACCCTCACAGGCTCCCTATCCTACCCAGCTCTTCCGAACTGTTCA
TGTAGTACAGCCAGTACAAGCTGTCCCAGAGGAAGCAACCATAGCCAGCACCATGCAGGAGGAAGCAGCA
AATTCTTCAGTTCCAAGTATTAGGACTATACAGGCTTCAACCCAGGTTCCAGTAGTGGTGTCTCCTGGCA
ACCAACAGTTACATACAGTAACAGTGCCACTCACAACGGTTATAGCCAGCATAGACCCATCATCAGGTGC
TGGGTCTCAGAAATTCATTTTACAAACCATTCCATCGTCACAGCCCATGACAGTACTGAAAGAAAATGTC
ATGCTACAGTCACAGAAGCCAGGCTCTCCTTCAATTGTCCTCAGCCCCACCCAAGTACAGCAGGTCCTCA
CAAGCAATGTTCAGTCCATTTGCAATGGAGCGGGCAGTGTGGCATCCGCACCATCGTTCAGCGCGACGAC
ACCTGTGGTGACTTTCTCTCGGAGTTCACAGCTGGTCGCACACCCACCGGGCACTGTGATCACTTCTGTT
ATCAAAGCTCAGGAAACAAAAACTCTTAAGCAAGAGGTGGAGAAAAAGGCCGAAGATGATTTGAATGAAG
ATGCTGAGAAAAGTGCCCAGCAGCCCCAGCCTTATGTGATGGTGTTATCCAGTTCAAACGGGTTTTCCTC
TCAGGTAGCCGTCAAACAGAATGAACTGCTAGAGCCCAACTCTTTTTAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TATTACCAAAGAGGTATTCTT; Reverse Primer - name:unspecified, sequence:TTAAAAAGAGTTGGGCTCTAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_019460 same experiment
 EMAGE:21712 same embryo
 EMAGE:21713 same embryo
 EMAGE:21714 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS