Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21713

Elf4 E74-like factor 4 (ets domain transcription factor) ( MGI:1928377)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713
"Pseudo-wholemount" of euxassay_019462. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019462_01 euxassay_019462_02 euxassay_019462_03 euxassay_019462_04
EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713
euxassay_019462_05 euxassay_019462_06 euxassay_019462_07 euxassay_019462_08 euxassay_019462_09
EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713
euxassay_019462_10 euxassay_019462_11 euxassay_019462_12 euxassay_019462_13 euxassay_019462_14
EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713
euxassay_019462_15 euxassay_019462_16 euxassay_019462_17 euxassay_019462_18 euxassay_019462_19
EMAGE:21713 EMAGE:21713 EMAGE:21713 EMAGE:21713
euxassay_019462_20 euxassay_019462_21 euxassay_019462_22 euxassay_019462_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21713Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21713_wholemount_strong.wlz
21713_wholemount_moderate.wlz
21713_wholemount_weak.wlz
21713_wholemount_possible.wlz
21713_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21713_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pericardium
moderate moderate
spottedmoderate expression: see section 3 4 5 weak expression: see section 9 0
mesenchyme
moderate moderate
regionalmoderate expression: see section 1 2 weak expression: see section 3 4 5 6 7 8 3 4 5 6 7 8 9 0 1 2 3
pancreas
moderate moderate
regionalmoderate expression: see section 6 7 8 9 weak expression: see section 5
vibrissa
weak weak
regionalweak expression: see section 4 5 6 1 2
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 3 4 weak expression: see section 2
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 2 3 4 5 weak expression: see section 0 1
stomach
moderate moderate
regionalmoderate expression: see section 8 weak expression: see section 9 0 1 2
hindgut
strong strong
regionalstrong expression: see section 4 moderate expression: see section 5
midgut
strong strong
regionalstrong expression: see section 8 9 0 3 4 moderate expression: see section 7 1 2 5 6 7 8 weak expression: see section 9
mandible
moderate moderate
regionalmoderate expression: see section 8 weak expression: see section 2 3 4 5 6 7 9 0 1 8 9 0 2 3
maxilla
moderate moderate
regionalmoderate expression: see section 8 weak expression: see section 3 4 5 6 7 9 0 1 8 9 0 2
bladder
weak weak
regionalweak expression: see section 2 3 4 5 6
lung
moderate moderate
spottedmoderate expression: see section 8 0 7 0 1 2 weak expression: see section 7 8 9
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55038
Entity Detected:Elf4, E74-like factor 4 (ets domain transcription factor) ( MGI:1928377)
Sequence:sense strand is shown

>T55038
TCGACCAGTACCATCCGGAGAGCCAGCTCCAGGGTCGGAACCAGAGCCTCCCCCGAGGACAAGGATAGCC
CTCCCTGGGAGAAGCCAAAAGTCCAACACACTGGTCTCCAGCCATCTGCAAGCCTGGAGTTAGGATTGTC
TGTGGATGAGGAGGTTCCCACCACCTCCACCATGCTTGCCAGCCCACTACAGAGCCAGGCCAGGCTCACC
AAAACTGTGAGTTCTTCTCCAGCACCCAGTAACATCCACCTAGGAGTAGCCCCTGTGGGCCCTGGCTCCA
CTGTCACCCTGCAGACCATCCCGCTGACTACGGTGCTGACCAATGGGCCTCCTGCCAGTACTACTGCTCC
AACTCAACTCGTTCTTCAGAGTGTCCCGGCAGTGTCAACATTCAAGGACACCTTCACTTTGCAGACCTCC
TTCCCCCTGAACACCAATTTGCAAGAGAACCAGGTGGCAACCCAAGGGGCCCCACTGATCCTCAGTGGTC
TCCCCCAACTTCTGGCTGGGGCCAACCCTCAGACGAACCCAGCTCCATCCCAGGTCATAGGTGCTGGATC
AGCAGGGCCTAGCTCTCAGCCCCCTGGGACTGTGATTGCTGCCTTTATCAGGACTTCCAGTGGCACATCA
GTGCCTGTGGTAAAGGAGGGGCCCTTGAGGTCCTCTTCATATGTGCAGGGTGTGGTTACTGGGGCACCCG
TGGAGGGGCTGTTGGTTCCTGAAGAGACCCTGAGAGAGCTCCTGAGAGATCAGGGTCATCTTCAGCCACT
TCCGAGTCAGGTGCTCTCAAGGGGTTCCCATAACCTGAGCCTTGTGGGGAACCAGACTCTCTCTCCCCCT
AGCCACCCCACTGTTGGGCTAACCCCAGTGGCTGAGCTCGAGCTCTCGTCAGGCTCAGGGCCCCTGTTCG
TAACTGAGCCTAGTGTGACCAGATCCCCAACCCAGGCCCCTTTCTCCCCATTCAATCCCACTTCCCTCAT
TAAGATGGAACCCCAAGATATATAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCGACCAGTACCATCCGGAGA; Reverse Primer - name:unspecified, sequence:TTATATATCTTGGGGTTCCAT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_019462 same experiment
 EMAGE:21711 same embryo
 EMAGE:21712 same embryo
 EMAGE:21714 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS