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EMAGE:21719

Cyp26b1 cytochrome P450, family 26, subfamily b, polypeptide 1 ( MGI:2176159)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719
"Pseudo-wholemount" of euxassay_018244. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018244_01 euxassay_018244_02 euxassay_018244_03 euxassay_018244_04
EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719
euxassay_018244_05 euxassay_018244_06 euxassay_018244_07 euxassay_018244_08 euxassay_018244_09
EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719
euxassay_018244_10 euxassay_018244_11 euxassay_018244_12 euxassay_018244_13 euxassay_018244_14
EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719
euxassay_018244_15 euxassay_018244_16 euxassay_018244_17 euxassay_018244_18 euxassay_018244_19
EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719 EMAGE:21719
euxassay_018244_20 euxassay_018244_21 euxassay_018244_22 euxassay_018244_23 euxassay_018244_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21719Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21719_wholemount_strong.wlz
21719_wholemount_moderate.wlz
21719_wholemount_weak.wlz
21719_wholemount_possible.wlz
21719_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21719_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50281
Entity Detected:Cyp26b1, cytochrome P450, family 26, subfamily b, polypeptide 1 ( MGI:2176159)
Sequence:sense strand is shown

>T50281
CAGAAGGAAGTCTGGGCTTGGGGCTTTGATGGCGGAGGATGGGAAGAGTCACTTCTATAAAATAATTCCT
CCTGGGCAAAGTGTTTGGAGCCAAGGCCTCCCCAGGCAATCTTTTTCCTCTCTCTCTTCGATCACCTCGC
AGCCTCCAGCCCCTTCCCCTCGGCCCCATCTCCTGGGTTTCTCCGAGGGGTGCCAGGACCCCCTGCCGCA
GCCTCCCCTCCCCCGTCACTCTGGTCCGGCCCTCCCCTCCCCCATCGCGGACCGCACAGCCAATCCCCCA
AGCGGCTGCCAACATGCTGTTTGAAGGCTTGGAGTTGGTGTCGGCGCTGGCCACCCTCGCCGCGTGCCTG
GTGTCCGTGACGCTGCTGCTGGCGGTGTCGCAGCAGCTGTGGCAGCTGCGCTGGGCTGCCACCCGCGACA
AGAGCTGCAAGCTGCCCATCCCCAAGGGCTCCATGGGATTCCCGCTCATCGGAGAGACTGGTCACTGGTT
GCTACAGGGTTCCGGCTTCCAGTCGTCGCGCCGGGAGAAGTATGGCAACGTTTTCAAGACACACTTACTG
GGGCGGCCGCTGATTCGTGTGACCGGTGCGGAGAATGTGCGCAAGATCCTACTGGGCGAACACCAGCTAG
TGAGCACCGAGTGGCCGCGGAGCGCACGCGTACTGCTGGGTCCCAACACGGTGGCCAATTCCATTGGCGA
CATCCACCGCAACAAGCGCAAGGTCTTCTCCAAGATCTTCAGCCATGAGGCACTTGAGAGCTACCTGCCC
AAGATCCAACTGGTGATCCAGGATACACTTCGAGCCTGGAGCAGCCAGCCTGAGGCCATCAATGTATATC
AGGAGGCCCAGCGACTTACCTTCCGAATGGCCGTGCGTGTGCTGCTAGGCTTCAGCATCCCTGAGGAGGA
CCTGGGCCACCTCTTTGAGGTATACCAGCAGTTTGTGGAGAATGTCTTCTCTCTGCCAGTGGACC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGAAGGAAGTCTGGGCTTG; Reverse Primer - name:unspecified, sequence:GGTCCACTGGCAGAGAGAAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_018244 same experiment
 EMAGE:21720 same embryo
 EMAGE:21721 same embryo
 EMAGE:21718 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS