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EMAGE:21722

Psen1 presenilin 1 ( MGI:1202717)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722
"Pseudo-wholemount" of euxassay_018240. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018240_01 euxassay_018240_02 euxassay_018240_03 euxassay_018240_04
EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722
euxassay_018240_05 euxassay_018240_06 euxassay_018240_07 euxassay_018240_08 euxassay_018240_09
EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722
euxassay_018240_10 euxassay_018240_11 euxassay_018240_12 euxassay_018240_13 euxassay_018240_14
EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722
euxassay_018240_15 euxassay_018240_16 euxassay_018240_17 euxassay_018240_18 euxassay_018240_19
EMAGE:21722 EMAGE:21722 EMAGE:21722 EMAGE:21722
euxassay_018240_20 euxassay_018240_21 euxassay_018240_22 euxassay_018240_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21722Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21722_wholemount_strong.wlz
21722_wholemount_moderate.wlz
21722_wholemount_weak.wlz
21722_wholemount_possible.wlz
21722_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21722_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50871
Entity Detected:Psen1, presenilin 1 ( MGI:1202717)
Sequence:sense strand is shown

>T50871
CGCACTACTGGACTGTGGAAGGAAGCGTCTACAGAGAACGGTTTCCAACATCCATCGCTGCAGCAGACGG
TGTCCCTCAGTGACTTGAGAGACAAGGACAAGGAAATGTGCTGGGCCAAGGAGCTGCCGTGCTCTGCTAG
CTTTGACCCGTGGGCATGGAGATTTACCCGCACTGTGAACTCTCTAAAGGTTAAACAAAGTGAGGTGAAC
CAAACAGAGCTGCCATCTTCCACACCATGTTGGAAATAAAACACGTCCTAGCTGAACCCTTACTGTCCAG
GAAGTTCCGTGTGGAAGTGGCACTGGGCCGGGCCTCCCTCTCAGGCTCCTTTGCTGCCCACTTGTAAGTT
TAAATAAGGACACCGCCCTACACAAACCTCACCCTGTCACATCAGTGACTCTGACCACTTTTGTTCTCAA
ACTCTCTCACTATTATCTGTGGTTGCCGTTTCTTCCCAAGGCCAGCCTGGACGAATTTGGGGTTGCTCTA
TCCTGAGAGTTGTAACCTCAACTTCCAAAGTTTATATTTTCTTGAAATGATGGATCTATTGCTCAACAGT
CCCTGTCATCCTTAAGTGACTTCTGGGTTTCCCACAAATTCCTCACTTTTAGACACACTCTAAGCTTACT
TCTGGCCTGNATGCTTCCTCTCCCTGTCTCTCCCTTGCCCCACAGCGGTTCCCTGACAGCAGACAAGGCA
GCTCTGGGAGGTAGCTAGTATCCAATAACCCAGGGGTTTCCTCATGTGATGCAAATACTACGTGTCCAAC
CAATCAGTGCTGTCAACGGGCTGCCATAGCTCCTTCGATGGCAAATAGGATGTGTGCCCAAAGAATTAAA
GCGATGAGTGGCTGGTGAAGCGCTCTCTGTCCTGGCTGGTTTT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CGCACTACTGGACTGTGGAA; Reverse Primer - name:unspecified, sequence:AAAACCAGCCAGGACAGAGA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_018240 same experiment
 EMAGE:21723 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS