EMAGE mouse gene expression spatial database

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EMAGE:21749

Tbx18 T-box18 ( MGI:1923615)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000121_01	euxassay_000121_02	euxassay_000121_03	euxassay_000121_04	euxassay_000121_05

euxassay_000121_06	euxassay_000121_07	euxassay_000121_08	euxassay_000121_09	euxassay_000121_10

euxassay_000121_11	euxassay_000121_12	euxassay_000121_13	euxassay_000121_14	euxassay_000121_15

euxassay_000121_16	euxassay_000121_17	euxassay_000121_18	euxassay_000121_19	euxassay_000121_20

euxassay_000121_21	euxassay_000121_22	euxassay_000121_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21749_wholemount_strong.wlz

21749_wholemount_moderate.wlz

21749_wholemount_weak.wlz

21749_wholemount_possible.wlz

21749_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21749_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

strong

regional

strong expression: see section 0 1 2 3

vibrissa

moderate

regional

moderate expression: see section 4 5 6 7 8 9 0 1 2 3

vibrissa dermal component

moderate

regional

moderate expression: see section 4 5 6 7

vibrissa epidermal component

moderate

regional

moderate expression: see section 7 8

inner ear

strong

regional

strong expression: see section 1

labyrinth

strong

regional

strong expression: see section 2 3 4 5 6 7 8 9 8 9 0 1 2 3

otic capsule

strong

regional

strong expression: see section 3 4 5 6 7 8 9 0 1 2 8 9 0 1 2 3

inner ear vestibular component

strong

regional

strong expression: see section 3 4 5 6 7 9 0 1 2 3

inner canthus

strong

graded

strong expression: see section 1 2

upper eyelid

strong

regional

strong expression: see section 1 2

perioptic mesenchyme

strong

regional

strong expression: see section 3

lower lip

moderate

regional

moderate expression: see section 7 8 9 0

upper lip

moderate

regional

moderate expression: see section 6 7

glans of male genital tubercle

moderate

regional

moderate expression: see section 0 1 2 5 weak expression: see section 6

urethra of male

strong

regional

strong expression: see section 2 6

vault of skull

moderate

regional

moderate expression: see section 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1527
Entity Detected:	Tbx18, T-box18 ( MGI:1923615)
Sequence:	sense strand is shown >T1527 TCCGCTCAGCAGTGGGGGCCAGCAGAGTTTCTTTGACTCCAGGACCCTAGGAAGTTTAACTCTGCCATCT CCTCAAGTGTCTGCACATATGGTCTGATGAAGCCTTTAAGTTAAACGGCGTTGGAATCTAGTGGATCTTC CTTATTTTTTTAAAGCAATATAGGGAAAATGTACACATAGTAGGAAAGGAAGGCCCAGTGAGTGTTTTTA CTTTCTCATGGCAAGATTGTAATTATGGTACATATGTATGCTGTATATATACATAGCATGTAGTATACTG AAGCCTTCTCTCTGCCTTGTCTATTCAGTTCCACAGAGTATTGGCGACATGGACTATGTTTATCCAGAAT AATCAGACTCTAAAAGTAAAATGGTACACTTGAAAGTTGGTAAGGATGCTACCAAAGACTTTTCTAATGC CCACGTGCCAAGTGATGGGGGTTTATTACAGCAGTGTCATACATACCTAACCAAAGATTTGTAACTTCAT TTATCAGAAGAATGCTCTGAGCAGTCTGT
Notes:	The probe template was PCR amplified from IMAGE:819918 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:819918 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000121 same experiment
	EMAGE:21747 same embryo
	EMAGE:21751 same embryo
	EMAGE:21750 same embryo
	EMAGE:21748 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source