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EMAGE:21795

Calca calcitonin/calcitonin-related polypeptide, alpha ( MGI:2151253)
TS13 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:21795 EMAGE:21795
Lateral view Transverse View of Midbrain

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21795Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21795_wholemount__moderate.wlz
21795_wholemount__weak.wlz
21795_wholemount__notDetected.wlz
21795_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21795_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
strong strong
regionalThere is strong expression in the anterior ventral neural tube.
notochord
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:691217
Entity Detected:Calca, calcitonin/calcitonin-related polypeptide, alpha ( MGI:2151253)
Notes:The Calca probe template used in this study by Tamplin et al, was PCR amplified from IMAGE:1331673 clone using the vector (pT7T3D) specific primers: pT7T3D FWD GTTTTCCCAGTCACGACGTT and pT7T3D REV TGTGGAATTGTGAGCGGATA. T3 polymerase was used to generate antisense probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.5 dpc
Theiler Stage:TS13
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Brian J. Cox, Janet Rossant.
Principal investigator:Janet Rossant, The Hospital for Sick Children Research Institute, TMDT at MaRS Room 13-305 101 College Street, Toronto, ON, Canada M5G1L7
Submitted by:Owen Tamplin, HHMI/Children's Hospital Boston, Zon Lab, Division of Hematology/Oncology 300 Longwood Avenue, Karp 8, Boston, MA, USA 02115
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2011.10.002] [ PMID:22008794] Tamplin OJ, Cox BJ, Rossant J 2011 Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord. Dev Biol (360)
Links:MGI:5291681 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE