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EMAGE:21801

Clcn2 chloride channel 2 ( MGI:105061)
TS12 (8.0 dpc)
in situ hybridisation

Data Images
EMAGE:21801 EMAGE:21801 EMAGE:21801
Lateral view Posterior Ventral Detail View Posterior View

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21801Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21801_wholemount__moderate.wlz
21801_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21801_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
hindgut diverticulum
detected detected
Expression is stronger in the hindgut and posterior notochord than elsewhere in the embryo.
notochord
detected detected
regionalExpression is stronger in the hindgut and posterior notochord than elsewhere in the embryo.
embryo
detected detected
Expression is widespread.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1375342
Entity Detected:Clcn2, chloride channel 2 ( MGI:105061)
Notes:The Clcn2 probe template used in this study by Tamplin et al, was PCR amplified from IMAGE:3492846 clone using the vector (pCMV-SPORT6) specific primers: pCMV-SPORT6 FWD ACAAAGATCCCAAGCTAGCAG and pCMV-SPORT6 REV TTGACCTCCATAGAAGACACC. T7 polymerase was used to generate antisense probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.0 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Brian J. Cox, Janet Rossant.
Principal investigator:Janet Rossant, The Hospital for Sick Children Research Institute, TMDT at MaRS Room 13-305 101 College Street, Toronto, ON, Canada M5G1L7
Submitted by:Owen Tamplin, HHMI/Children's Hospital Boston, Zon Lab, Division of Hematology/Oncology 300 Longwood Avenue, Karp 8, Boston, MA, USA 02115
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2011.10.002] [ PMID:22008794] Tamplin OJ, Cox BJ, Rossant J 2011 Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord. Dev Biol (360)
Links:MGI:5291780 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE